中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2015年
5期
577-579
,共3页
梁桦%先杰%黄翔%徐枫%张涛%杨承祥
樑樺%先傑%黃翔%徐楓%張濤%楊承祥
량화%선걸%황상%서풍%장도%양승상
麻醉药,吸入%肺肿瘤%肿瘤转移%肝素裂合酶%细胞运动
痳醉藥,吸入%肺腫瘤%腫瘤轉移%肝素裂閤酶%細胞運動
마취약,흡입%폐종류%종류전이%간소렬합매%세포운동
Anesthetics,inhalation%Lung neoplasms%Neoplasm metastasis%Heparin lyase%Cell movement
目的:评价七氟醚对小鼠肺癌细胞乙酰肝素酶( HPA)及Fascin表达的影响。方法小鼠Lewis肺癌细胞接种于培养板,培养24 h后,采用随机数字表法分为4组( n=18):对照组( C组)、1%七氟醚组( Sev1组)、2%七氟醚组( Sev2组)和3%七氟醚组( Sev3组)。 C组不接受七氟醚处理,Sev1?3组细胞分别用1%、2%和3%七氟醚处理4 h,继续培养24 h。采用Transwell法检测侵袭细胞数,细胞划痕实验检测细胞迁移率;采用Western blot法检测HPA和Fascin表达。结果与C组比较,Sev1?3组侵袭细胞数和细胞迁移率依次降低,HPA和Fascin表达依次下调( P <0.05)。结论七氟醚抑制小鼠肺癌细胞转移能力的机制与下调HPA和Fascin表达有关。
目的:評價七氟醚對小鼠肺癌細胞乙酰肝素酶( HPA)及Fascin錶達的影響。方法小鼠Lewis肺癌細胞接種于培養闆,培養24 h後,採用隨機數字錶法分為4組( n=18):對照組( C組)、1%七氟醚組( Sev1組)、2%七氟醚組( Sev2組)和3%七氟醚組( Sev3組)。 C組不接受七氟醚處理,Sev1?3組細胞分彆用1%、2%和3%七氟醚處理4 h,繼續培養24 h。採用Transwell法檢測侵襲細胞數,細胞劃痕實驗檢測細胞遷移率;採用Western blot法檢測HPA和Fascin錶達。結果與C組比較,Sev1?3組侵襲細胞數和細胞遷移率依次降低,HPA和Fascin錶達依次下調( P <0.05)。結論七氟醚抑製小鼠肺癌細胞轉移能力的機製與下調HPA和Fascin錶達有關。
목적:평개칠불미대소서폐암세포을선간소매( HPA)급Fascin표체적영향。방법소서Lewis폐암세포접충우배양판,배양24 h후,채용수궤수자표법분위4조( n=18):대조조( C조)、1%칠불미조( Sev1조)、2%칠불미조( Sev2조)화3%칠불미조( Sev3조)。 C조불접수칠불미처리,Sev1?3조세포분별용1%、2%화3%칠불미처리4 h,계속배양24 h。채용Transwell법검측침습세포수,세포화흔실험검측세포천이솔;채용Western blot법검측HPA화Fascin표체。결과여C조비교,Sev1?3조침습세포수화세포천이솔의차강저,HPA화Fascin표체의차하조( P <0.05)。결론칠불미억제소서폐암세포전이능력적궤제여하조HPA화Fascin표체유관。
Objective To evaluate the effects of sevoflurane on the expression of heparanase ( HPA) and fascin in lung carcinoma cells of mice. Methods Mouse LLC cells were inoculated in the culture plate. After being cultured for 24 h, the cells were equally and randomly divided into 4 groups using a random number table: control group ( group CC) , 1% sevoflurane group ( group Sev1 ) , 2% sevoflurane group ( group Sev2 ) , and 3% sevoflurane group ( group Sev3 ) . Cells in Sev1-3 groups were exposed to 1%, 2% and 3% sevoflurane, respectively, for 4 h, while cells in group CC were not exposed to sevoflurane, and all the cells were then cultured for another 24 h in an incubator. The invasion of cells was determined by Transwell invasion assay, and the invaded cells were counted. The migration of cells was determined by wound healing assay, and cell migration rates were calculated. The expression of HPA and fascin in cells was detected by Western blot. Results Compared with group CC, the number of invaded cells and cell migration rates were gradually decreased, and the expression of HPA and fascin was gradually down?regulated with increasing concentrations of sevoflurane in Sev1-3 groups. Conclusion The mechanism through which sevoflurane inhibits the metastasis of mouse lung carcinoma cells is associated with down?regulated expression of HPA and fascin.