CAJ | 학술논문

叶酸和维生素B12对胎儿生长受限大鼠胰岛素抵抗的影响
협산화유생소B12대태인생장수한대서이도소저항적영향
Effects of folic acid and vitamin B12 on insulin resistance of fetal growth restriction rats

万方数据

中华围产医学杂志中華圍產醫學雜誌 중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2015年 8期 589-593 ,共5页

张慧%邢燕%张金%王新利%朴梅花%童笑梅%韩彤妍

장혜%형연%장금%왕신리%박매화%동소매%한동연

胎儿生长迟缓%胰岛素抗药性%叶酸%维生素B12%大鼠,Sprague-Dawlev 胎兒生長遲緩%胰島素抗藥性%葉痠%維生素B12%大鼠,Sprague-Dawlev
태인생장지완%이도소항약성%협산%유생소B12%대서,Sprague-Dawlev
Fetal growth retardation%Insulin resistance%Folic acid%Vitamin B12%Rats,Sprague-Dawley

目的探讨在哺乳期母鼠的食物中添加叶酸和维生素B12对胎儿生长受限(fetal growth restriction,FGR)仔鼠胰岛素抵抗的影响. 方法 18只Sprague-Dawley雌鼠与雄鼠交配,受孕第12天随机分为低蛋白组(12只)和对照组(6只).孕鼠自然分娩,低蛋白组中FGR仔鼠共84只(93.3％,84/90),随机抽取48只分为FGR干预组和FGR未干预组(各24只).干预组的哺乳期母鼠饲以高叶酸和维生素B12的饲料,未干预组和对照组的哺乳期母鼠饲以普通饲料.3组仔鼠均于生后21d断奶,饲以普通饲料.于仔鼠生后21、60和120 d,每组随机选择8只仔鼠,测定血浆葡萄糖(简称血糖)、血清胰岛素、甘油三酯和胆固醇水平,并计算胰岛素敏感指数(insulin sensitivity index,ISI)和胰岛素抵抗指数(insulin resistance index,IRI).采用方差分析、Student-Newman-Keuls法进行统计分析. 结果 (1)FGR发生率:低蛋白组仔鼠平均出生体重[(4.44±0.58)g]低于对照组[(7.03±0.56)g],差异有统计学意义(t=15.75,P＜0.05).(2)血糖和胰岛素水平:生后21d,FGR未干预组、干预组和对照组的空腹血糖(fasting plasmaglucose,FPG)分别为(4.8±0.3)、(4.8±0.4)与(4.6±0.3)mmol/L (F=0.57),空腹胰岛素(fasting insulin,FINS)分别为(4.2±0.2)、(4.5±0.4)与(4.3±0.1)mU/L(F=0.31),差异均无统计学意义(P值均＞ 0.05).生后60 d,3组FPG分别为(7.5±0.4)、(6.9±1.0)与(5.5±0.6) mmol/L(F=17.14),FINS分别为(14.7±1.9)、(10.7±1.0)与(9.2±0.7) mU/L(F=38.34);生后120 d,3组FPG分别为(8.9±0.9)、(8.0±0.8)与(6.4±0.7)mmol/L(F=21.60),FINS分别为(56.3±2.8)、(38.2±2.5)与(33.1±2.8)mU/L(F=164.46);FGR未干预组最高,对照组最低,差异均有统计学意义(P值均＜ 0.05).(3)IRI和ISI:生后21d,FGR未干预组、干预组和对照组的IRI分别为0.9±0.1、0.9±0.1与0.9±0.2(F=0.49),ISI分别为－(3.0±0.7)、-(3.0±0.1)与-(3.0±0.3)(F=0.69),差异均无统计学意义(P值均＞0.05).生后60d,3组IRI分别为4.9±0.8、3.3±0.3与2.2±0.3(F=49.48),ISI分别为－(4.7±0.2)、－(4.3±0.1)与－(3.9±0.1) (F=63.47);生后120 d,3组IRI分别为22.4±3.1、13.6±2.0与9.3±0.9(F=75.15),ISI分别为－(6.2±0.1)、-(5.7±0.1)与－(5.3±0.1)(F=104.42),差异均有统计学意义(P值均＜ 0.05).(4)胆固醇和甘油三酯:生后21 d,FGR未干预组、干预组和对照组的胆固醇分别为(2.0±0.1)、(2.0±0.1)与(2.0±0.1)mmol/L (F=0.10),甘油三酯分别为(0.8±0.1)、(0.8±0.1)与(0.7±0.1)mmol/L(F=0.33),差异均无统计学意义(P值均＞0.05).生后60 d,3组胆固醇分别为(2.3±0.1)、(2.2±0.1)与(2.0±0.2) mmol/L(F=8.34),甘油三酯分别为(1.5±0.2)、(1.2±0.1)与(1.0±0.2) mmol/L (F=17.93);生后120 d,3组胆固醇分别为(2.4±0.2)、(2.2±0.1)与(2.1±0.1) mmol/L (F=6.12),甘油三酯分别为(1.7±0.5)、(1.2±0.3)与(1.0±0.1)mmol/L(F=9.80);FGR未干预组最高,对照组最低,差异均有统计学意义(P值均＜ 0.05). 结论母鼠哺乳期补充叶酸和维生素B12,能一定程度上改善FGR仔鼠的胰岛素抵抗,但并不足以完全修复糖脂代谢.
목적 탐토재포유기모서적식물중첨가협산화유생소B12대태인생장수한(fetal growth restriction,FGR)자서이도소저항적영향. 방법 18지Sprague-Dawley자서여웅서교배,수잉제12천수궤분위저단백조(12지)화대조조(6지).잉서자연분면,저단백조중FGR자서공84지(93.3％,84/90),수궤추취48지분위FGR간예조화FGR미간예조(각24지).간예조적포유기모서사이고협산화유생소B12적사료,미간예조화대조조적포유기모서사이보통사료.3조자서균우생후21d단내,사이보통사료.우자서생후21、60화120 d,매조수궤선택8지자서,측정혈장포도당(간칭혈당)、혈청이도소、감유삼지화담고순수평,병계산이도소민감지수(insulin sensitivity index,ISI)화이도소저항지수(insulin resistance index,IRI).채용방차분석、Student-Newman-Keuls법진행통계분석. 결과 (1)FGR발생솔:저단백조자서평균출생체중[(4.44±0.58)g]저우대조조[(7.03±0.56)g],차이유통계학의의(t=15.75,P＜0.05).(2)혈당화이도소수평:생후21d,FGR미간예조、간예조화대조조적공복혈당(fasting plasmaglucose,FPG)분별위(4.8±0.3)、(4.8±0.4)여(4.6±0.3)mmol/L (F=0.57),공복이도소(fasting insulin,FINS)분별위(4.2±0.2)、(4.5±0.4)여(4.3±0.1)mU/L(F=0.31),차이균무통계학의의(P치균＞ 0.05).생후60 d,3조FPG분별위(7.5±0.4)、(6.9±1.0)여(5.5±0.6) mmol/L(F=17.14),FINS분별위(14.7±1.9)、(10.7±1.0)여(9.2±0.7) mU/L(F=38.34);생후120 d,3조FPG분별위(8.9±0.9)、(8.0±0.8)여(6.4±0.7)mmol/L(F=21.60),FINS분별위(56.3±2.8)、(38.2±2.5)여(33.1±2.8)mU/L(F=164.46);FGR미간예조최고,대조조최저,차이균유통계학의의(P치균＜ 0.05).(3)IRI화ISI:생후21d,FGR미간예조、간예조화대조조적IRI분별위0.9±0.1、0.9±0.1여0.9±0.2(F=0.49),ISI분별위－(3.0±0.7)、-(3.0±0.1)여-(3.0±0.3)(F=0.69),차이균무통계학의의(P치균＞0.05).생후60d,3조IRI분별위4.9±0.8、3.3±0.3여2.2±0.3(F=49.48),ISI분별위－(4.7±0.2)、－(4.3±0.1)여－(3.9±0.1) (F=63.47);생후120 d,3조IRI분별위22.4±3.1、13.6±2.0여9.3±0.9(F=75.15),ISI분별위－(6.2±0.1)、-(5.7±0.1)여－(5.3±0.1)(F=104.42),차이균유통계학의의(P치균＜ 0.05).(4)담고순화감유삼지:생후21 d,FGR미간예조、간예조화대조조적담고순분별위(2.0±0.1)、(2.0±0.1)여(2.0±0.1)mmol/L (F=0.10),감유삼지분별위(0.8±0.1)、(0.8±0.1)여(0.7±0.1)mmol/L(F=0.33),차이균무통계학의의(P치균＞0.05).생후60 d,3조담고순분별위(2.3±0.1)、(2.2±0.1)여(2.0±0.2) mmol/L(F=8.34),감유삼지분별위(1.5±0.2)、(1.2±0.1)여(1.0±0.2) mmol/L (F=17.93);생후120 d,3조담고순분별위(2.4±0.2)、(2.2±0.1)여(2.1±0.1) mmol/L (F=6.12),감유삼지분별위(1.7±0.5)、(1.2±0.3)여(1.0±0.1)mmol/L(F=9.80);FGR미간예조최고,대조조최저,차이균유통계학의의(P치균＜ 0.05). 결론 모서포유기보충협산화유생소B12,능일정정도상개선FGR자서적이도소저항,단병불족이완전수복당지대사.
Objective To explore the effects of folic acid and vitamin B12 supplement in maternal lactation on insulin resistance in fetal growth restriction (FGR) in rat offspring.Methods Eighteen Sprague-Dawley female rats and male rats were used.Pregnant rats were randomly divided into two groups at 12 days:normal-protein group (NP,n=6) and low-protein group (LP,n=12).The were 84 FGR newborn pups in LP group (93.3％,84/90).Forty-eight FGR newborn pups were randomly selected and divided into two groups (24 in each group):intervention group and non-intervention group.The intervention group was fed with high folate and vitamin B12 in the diet;and non-intervention group and NP group were fed normal diet.All of the newborn pups were weaned at 21 days after birth and then fed with normal diet.At days 21,60 and 120 d after birth,eight pups were randomly selected from each group and fasting plasma glucose (FPG),fasting insulin (FINS),blood diglyceride (TG) and cholesterol (TC) were measured.The insulin resistance index (IRI) and insulin sensitivity index (ISI) were calculated to evaluate insulin sensitivity.Variance and Student-Newman-Keuls tests were used for statistical analysis.Results (1) The incidence of FGR:Birth weight of LP offspring [(4.44±0.58) g] was significantly lower than that of NP ones [(7.03±0.56) g] (t=15.75,P ＜ 0.05).(2) FPG and FINS:at day 21 after birth,FPG of the non-intervention group,intervention group and NP group was (4.8±0.3),(4.8±0.4) and (4.6±0.3) mmol/L (F=0.57),respectively;FINS was (4.2± 0.2),(4.5 ±0.4) and (4.3 ±0.1) mU/L (F=0.31),respectively;and there was no significant difference among the three groups (both P ＞ 0.05).At day 60,FPG of the three groups was (7.5±0.4),(6.9± 1.0) and (5.5±0.6) mmol/L (F=17.14);FINS was (14.7± 1.9),(10.7± 1.0) and (9.2± 0.7) mU/L (F=38.34),respectively.At day 120,FPG was (8.9±0.9),(8.0±0.8) and (6.4±0.7) mmol/L (F=21.60);FINS was (56.3±2.8),(38.2±2.5) and (33.1 ±2.8) mU/L (F=164.46).FPG and FINS were highest in the non-intervention group,and lowest in NP group,with significant differences among the three groups of pups (all P ＜ 0.05).(3) IRI and ISI:at day 21,IRI of the non-intervention group,intervention group and the control group was 0.9±0.1,0.9±0.1 and 0.9±0.2 (F=0.49);ISI was-(3.0±0.7),-(3.0±0.1) and-(3.0±0.3) (F=0.69);and there was no significant difference among the three groups (both P ＞ 0.05).At day 60,IRI of the three groups was 4.9±0.8,3.3±0.3 and 2.2±0.3 (F=49.48);ISI was-(4.7±0.2),-(4.3±0.1) and-(3.9±0.1) (F=63.47).At day 120,IRI of the three groups was 22.4±3.1,13.6±2.0 and 9.3±0.9 (F=75.15);ISI was -(6.2 ± 0.1),-(5.7 ± 0.1) and-(5.3 ± 0.1) (F=104.42);and there were significant differences among the three groups (all P ＜ 0.05).(4) TC and TG:at day 21,TC of the non-intervention group,intervention group and the control group was (2.0±0.1),(2.0±0.1) and (2.0±0.1) mmol/L (F=0.10);TG was (0.75±0.1),(0.77±0.1) and (0.74±0.1) mmol/L (F=0.33);and there was no significant difference among the three groups (both P ＞ 0.05).At day 60,TC of the three groups was (2.3 ± 0.1),(2.2 ± 0.1) and (2.0± 0.2) mmol/L (F=8.34);TG was (1.5 ± 0.2),(1.2±0.1) and (1.0±0.2) mmol/L (F=17.93).At day 120,TC was (2.4±0.2),(2.2±0.1) and (2.1 ±0.1) mmol/L (F=6.12);TG was (1.7±0.5),(1.2±0.3) and (l.0±0.1) mmol/L (F=9.80).The TC and TG were highest in the non-intervention group and the lowest in the control group;and there were significant differences among the three groups (all P ＜ 0.05).Conclusion Supplementing folic acid and vitamin B12 in maternal lactation can improve in some extent insulin resistance in FGR rats,but not sufficient enough to completely repair glucose and lipid metabolism.