CAJ | 학술논문

将解淀粉芽孢杆菌BI2发酵所产抑菌物质作为新型种衣剂的有效成分与研制的复合型成膜剂溶液混合,制成可抑制黄曲霉孢子萌发的花生种子包衣.结果表明,选用 4%,聚乙烯醇(PVA)与 1.5%,羧甲基纤维素钠(CMC-Na)以体积比 5:1 混合作为种子包衣最佳成膜剂配方,经过包衣后对花生种子的发芽势和发芽率没有显著影响.含有抑菌物质的发酵液冻干粉的 pH 稳定性和热稳定性均较好.发酵液冻干粉抑制黄曲霉孢子萌发的最小质量浓度为1.92,mg/mL.在与花生种子混合时,发酵液冻干粉在成膜剂中的含量达到4,mg/mL时,黄曲霉完全被抑制.
장해정분아포간균BI2발효소산억균물질작위신형충의제적유효성분여연제적복합형성막제용액혼합,제성가억제황곡매포자맹발적화생충자포의.결과표명,선용 4%,취을희순(PVA)여 1.5%,최갑기섬유소납(CMC-Na)이체적비 5:1 혼합작위충자포의최가성막제배방,경과포의후대화생충자적발아세화발아솔몰유현저영향.함유억균물질적발효액동간분적 pH 은정성화열은정성균교호.발효액동간분억제황곡매포자맹발적최소질량농도위1.92,mg/mL.재여화생충자혼합시,발효액동간분재성막제중적함량체도4,mg/mL시,황곡매완전피억제.
The antifungal substances produced from Bacillus amyloliquefaciens BI2,were for the first time used asactive ingredients in seed coating. The fermented broth containing antifungal substances was ultrafiltered and freeze-dried to form lyophilized powder,and then mixed with composite film-forming agent solution. Finally,peanut seeds were coated with this kind of seed coating containing antifungal substances. The results showed that the best volume ratio of 4%, polyvinyl alco-hol(PVA)to 1.5%, sodium carboxymethyl cellulose(CMC-Na)was 5:1,which is the best film former recipe in seed coating,and it has no significant effect on seed germination potential and germination rate. The pH stability andthermal stability of the lyophilized powder of the fermentated broth containing antifungal substances are good. The MIC of the pow-der to inhibitAspergillus flavus spore germination is 1.92,mg/mL. When the content of that powder in the film former reaches 4,mg/mL,A. flavuswas completely inhibited.