CAJ | 학술논문

目的：观察疫苗生产中使用的消毒剂酸性苯酚和碱性苯酚对M RC‐5细胞生长的影响。方法将酸性苯酚（0．37％）和碱性苯酚（0．74％）分别加入M RC‐5细胞培养瓶中，观察细胞生长形态，绘制细胞生长曲线，测定细胞贴壁率。分别采用 t检验和χ2检验对结果进行比较。结果加入酸性和碱性苯酚后，第7天的细胞数分别为6．65×105和7．12×105，与对照组（6．93×105）相比，差异无统计学意义（t＝0．476，P＝0．654；t＝0．334，P＝0．752）；第8小时的细胞贴壁率分别为88．3％和96．0％，与对照组（90．3％）相比，差异亦无统计学意义（χ2＝0．765，P＝0．659；χ2＝0．376，P＝0．585）。结论微量酸性和碱性苯酚对M RC‐5细胞的生长没有显著影响。
목적：관찰역묘생산중사용적소독제산성분분화감성분분대M RC‐5세포생장적영향。방법장산성분분（0．37％）화감성분분（0．74％）분별가입M RC‐5세포배양병중，관찰세포생장형태，회제세포생장곡선，측정세포첩벽솔。분별채용 t검험화χ2검험대결과진행비교。결과가입산성화감성분분후，제7천적세포수분별위6．65×105화7．12×105，여대조조（6．93×105）상비，차이무통계학의의（t＝0．476，P＝0．654；t＝0．334，P＝0．752）；제8소시적세포첩벽솔분별위88．3％화96．0％，여대조조（90．3％）상비，차이역무통계학의의（χ2＝0．765，P＝0．659；χ2＝0．376，P＝0．585）。결론미량산성화감성분분대M RC‐5세포적생장몰유현저영향。
Objective To investigate effect of acid phenol and alkaline phenol used in vaccine production as disinfectants on MRC‐5 cell growth .Methods Acid phenol(0 .37% ) and alkaline phenol (0 .74% )were added to MRC‐5 cell culture ,respectively .The morphology and growth curve of cells were observed ,and cell attachment rate determined .The results were compared using t‐test and χ2‐test . Results The number of MRC‐5 cells added with acid phenol (6 .65 × 105 ) and alkaline phenol (7 .12 × 105 ) had no difference from that of control cells (6 .93 × 105 ) at 7 d (t= 0 .476 ,P= 0 .654 ;t=0 .334 ,P=0 .752) .No difference of attachment rate was observed between the treated cells (88 .3%and 96 .0% ) and control cells (90 .3% ) at 8 h (χ2 = 0 .765 ,P= 0 .659 ;χ2 = 0 .376 ,P= 0 .585 ) . Conclusion Trace amount of acid and alkaline phenols has no significant effect on growth of MRC‐5 cells .