CAJ | 학술논문

目的：：探讨MCI-186对海人酸( KA)诱导癫痫大鼠海马组织细胞色素C( Cyt C)表达的影响。方法：SD大鼠随机分为癫痫对照组、癫痫组、药物对照组组和MCI-186组。采用脑室注射KA制作大鼠癫痫模型。应用Western-blot法检测细胞质和线粒体中Cyt C。结果：癫痫组和药物对照组Cyt C在线粒体的表达均较MCI-186组减少(P<0.01)，癫痫组和药物对照组Cyt C在细胞质的表达均较MCI-186组增加(P<0.01)。结论：MCI-186可以减少KA诱导Cyt C由线粒体向细胞质的释放，通过抑制线粒体凋亡通路对海马细胞发挥保护作用。
목적：：탐토MCI-186대해인산( KA)유도전간대서해마조직세포색소C( Cyt C)표체적영향。방법：SD대서수궤분위전간대조조、전간조、약물대조조조화MCI-186조。채용뇌실주사KA제작대서전간모형。응용Western-blot법검측세포질화선립체중Cyt C。결과：전간조화약물대조조Cyt C재선립체적표체균교MCI-186조감소(P<0.01)，전간조화약물대조조Cyt C재세포질적표체균교MCI-186조증가(P<0.01)。결론：MCI-186가이감소KA유도Cyt C유선립체향세포질적석방，통과억제선립체조망통로대해마세포발휘보호작용。
Objective:To investigate the effects of MCI-186 on the expression of cytochrome C(Cyt C) in hippocampus of epilepsy rats induced by kainic acid( KA) . Methods:The SD rats were randomly divided into the control group,seizure group,drug control group and MCI-186 group. The seizures model of rats were established by injecting KA into encephalocoele. The Cyt C expressions in cytoplasm and mitochondria were detected using Western-blot. Results:The Cyt C expressions in mitochondria in seizure group and drug control group were lower than that in MCI-186 group(P<0. 01). The Cyt C expressions in cytoplasm in seizure group and drug control group were higher than that in MCI-186 group(P<0. 01). Conclusions:MCI-186 may decrease the release of Cyt C from mitochondria to cytoplasm,and which can protect the hippocampus by inhibiting the mitochondrial apoptotic pathway.