CAJ | 학술논문

目的：：观察氯诺昔康联合地佐辛对切口痛大鼠模型的超前镇痛效应,探讨其可能作用机制。方法：40只Wistar大鼠随机分为空白对照组、切口痛模型组、氯诺昔康组、地佐辛组和氯诺昔康联合地佐辛组(联合用药组)各8只。空白对照组不做任何处理；切口痛模型组术前30 min尾静脉注射0.9%氯化钠注射液；氯诺昔康组术前30 min尾静脉注射氯诺昔康2 mg/kg；地佐辛组术前30 min尾静脉注射地佐辛5 mg；联合用药组术前30 min尾静脉注射氯诺昔康1 mg/kg,地佐辛2.5 mg。术后采用累计疼痛评分法给予切口痛大鼠疼痛评分,术后3h大鼠尾静脉采血,于脊髓膨大处取脊髓,分别检测血清白细胞介素( IL)-6和脊髓环氧化酶( COX)-2的含量。结果：切口痛模型组大鼠累计疼痛评分均明显高于氯诺昔康组、地佐辛组和联合用药组(P<0.01),而联合用药组大鼠累计疼痛评分均较单用药组显著下降(P<0.01)；联合用药组大鼠血清IL-6和脊髓COX-2的表达均显著低于单用药组(P<0.01)。结论：氯诺昔康联合地佐辛能通过抑制切口痛大鼠血清IL-6和脊髓COX-2的表达,降低大鼠累计疼痛评分,表现出显著的超前镇痛作用。
목적：：관찰록낙석강연합지좌신대절구통대서모형적초전진통효응,탐토기가능작용궤제。방법：40지Wistar대서수궤분위공백대조조、절구통모형조、록낙석강조、지좌신조화록낙석강연합지좌신조(연합용약조)각8지。공백대조조불주임하처리；절구통모형조술전30 min미정맥주사0.9%록화납주사액；록낙석강조술전30 min미정맥주사록낙석강2 mg/kg；지좌신조술전30 min미정맥주사지좌신5 mg；연합용약조술전30 min미정맥주사록낙석강1 mg/kg,지좌신2.5 mg。술후채용루계동통평분법급여절구통대서동통평분,술후3h대서미정맥채혈,우척수팽대처취척수,분별검측혈청백세포개소( IL)-6화척수배양화매( COX)-2적함량。결과：절구통모형조대서루계동통평분균명현고우록낙석강조、지좌신조화연합용약조(P<0.01),이연합용약조대서루계동통평분균교단용약조현저하강(P<0.01)；연합용약조대서혈청IL-6화척수COX-2적표체균현저저우단용약조(P<0.01)。결론：록낙석강연합지좌신능통과억제절구통대서혈청IL-6화척수COX-2적표체,강저대서루계동통평분,표현출현저적초전진통작용。
Objective:To observe the preemptive analgesia effects of lornoxicam combined with dezocine on the rat with incision pain, and investigate its possible mechanism. Methods:Forty Wistar rats were randomly divided into the control group ( without any treatment),incision pain model group(treatment with preoperative 30 min tail vein injection of saline),lornoxicam group(treatment with preoperative 30 min tail vein injection of 2 mg/kg of lornoxicam),dezocine group(treatment with preoperative 30min tail vein injection of 5 mg of lornoxicam) and lornoxicam combined with dezocine group( treatment with preoperative 30 min tail vein injection of 1 mg/kg of lornoxicam combined with 2. 5 mg of lornoxicam). The pain score of rats with incision pain after operation was investigated using the cumulative pain score method. The interleukin(IL)-6 expression in the rat tail vein and cyclooxygenase(COX)-2 level in spinal cord were measured after 3h of operation. Results:The cumulative pain score in incision pain model group was significantly higher than that in lornoxicam group,dezocine group and lornoxicam combined with dezocine group(P<0. 01). The cumulative pain score in lornoxicam combined with dezocine group was significantly lower than that in single drug group(P<0. 01). The expressions of serum IL-6 and COX-2 in spinal cord in lornoxicam combined with dezocine group were significantly lower than that in single drug group (P<0. 01). Conclusions:Lornoxicam combined with dezocine can inhibit the expressions of serum IL-6 and COX-2 in spinal cord and decrease the pain score in rat with incision pain,which plays the preemptive analgesia effects.