中国药品标准
中國藥品標準
중국약품표준
DRUG STANDARDS OF CHINA
2015年
4期
257-260
,共4页
注射用环磷腺苷葡胺%葡甲胺%高效液相色谱法%含量测定
註射用環燐腺苷葡胺%葡甲胺%高效液相色譜法%含量測定
주사용배린선감포알%포갑알%고효액상색보법%함량측정
Meglumine Adenosine Cyclophosphate for Injection%Meglumine%HPLC%determination
目的：：建立HPLC法测定注射用环磷腺苷葡胺中葡甲胺的含量。方法：XDB-C18柱(4．6 mm ×250 mm,5μm),流动相为庚烷磺酸钠缓冲液-乙腈(95∶5),流量为1．0 mL·min-1,检测波长为195 nm。结果：葡甲胺在0．25~5 mg·mL-1浓度范围内线性回归方程的相关系数为0．9999；平均回收率为100．1%,其RSD为0．42%。结论：本法操作简便,结果准确,可用于注射用环磷腺苷葡胺中葡甲胺的含量测定。
目的：：建立HPLC法測定註射用環燐腺苷葡胺中葡甲胺的含量。方法：XDB-C18柱(4．6 mm ×250 mm,5μm),流動相為庚烷磺痠鈉緩遲液-乙腈(95∶5),流量為1．0 mL·min-1,檢測波長為195 nm。結果：葡甲胺在0．25~5 mg·mL-1濃度範圍內線性迴歸方程的相關繫數為0．9999；平均迴收率為100．1%,其RSD為0．42%。結論：本法操作簡便,結果準確,可用于註射用環燐腺苷葡胺中葡甲胺的含量測定。
목적：：건립HPLC법측정주사용배린선감포알중포갑알적함량。방법：XDB-C18주(4．6 mm ×250 mm,5μm),류동상위경완광산납완충액-을정(95∶5),류량위1．0 mL·min-1,검측파장위195 nm。결과：포갑알재0．25~5 mg·mL-1농도범위내선성회귀방정적상관계수위0．9999；평균회수솔위100．1%,기RSD위0．42%。결론：본법조작간편,결과준학,가용우주사용배린선감포알중포갑알적함량측정。
Objective:To establish an HPLC method for determination of Meglumine in Meglumine Adenosine Cyclophosphate for In-jection. Methods:The determination was performed on a XDB-C18 column(4. 6 mm × 250 mm,5 μm) with mobile phase of sodium heptanesulfonate buffer solution-acetonitrile (95∶5) . The flow rate was 1. 0 mL·min-1 and the detection wavelength was 195 nm. Re-sults:Meglumine showed a good linearity in the range of 0. 25-5 mg·mL-1(r=0. 999 9), the average recovery was 100. 1% with RSD of 0. 42%. Conclusion:The method is simple and accurate which could be used to determine Meglumine in Meglumine Adeno-sine Cyclophosphate for Injection .
