CAJ | 학술논문

SBP转录因子家族作为植物特异性的转录因子具有重要的生物学功能，广泛参与花和果实发育等诸多生命过程。利用普通烟草（Nicotiana tabacum）TN90基因组数据，通过隐马尔科夫模型检索，SMART和Pfam分析，鉴定了普通烟草SBP转录因子家族成员；通过多序列比对鉴定并分析了普通烟草 SBP 结构域的结构特征；通过邻接法、极大似然法进行了系统发育分析；分别利用 GSDS 和 MEME 对 SBP 转录因子家族成员的基因结构和蛋白保守结构域进行了分析；利用普通烟草TN90转录组数据分析了SBP转录因子家族成员在不同组织和时期的表达情况，并对鉴定出的SBP转录因子家族成员进行了GO注释分析。结果表明，普通烟草基因组编码32个SBP转录因子家族成员，氨基酸序列长度差异较大；系统发育分析表明，所有的SBP转录因子家族成员可分为8个亚家族。转录组数据显示，SBP基因家族成员在不同组织和时期中表达迥异，但在花器官中均有较高的表达量。GO注释结果表明，该家族成员作为转录因子，在植物的生长发育、逆境响应等过程中发挥作用。本研究为烟草及其他植物中SBP转录因子家族基因的鉴定和功能研究提供了基础。
SBP전록인자가족작위식물특이성적전록인자구유중요적생물학공능，엄범삼여화화과실발육등제다생명과정。이용보통연초（Nicotiana tabacum）TN90기인조수거，통과은마이과부모형검색，SMART화Pfam분석，감정료보통연초SBP전록인자가족성원；통과다서렬비대감정병분석료보통연초 SBP 결구역적결구특정；통과린접법、겁대사연법진행료계통발육분석；분별이용 GSDS 화 MEME 대 SBP 전록인자가족성원적기인결구화단백보수결구역진행료분석；이용보통연초TN90전록조수거분석료SBP전록인자가족성원재불동조직화시기적표체정황，병대감정출적SBP전록인자가족성원진행료GO주석분석。결과표명，보통연초기인조편마32개SBP전록인자가족성원，안기산서렬장도차이교대；계통발육분석표명，소유적SBP전록인자가족성원가분위8개아가족。전록조수거현시，SBP기인가족성원재불동조직화시기중표체형이，단재화기관중균유교고적표체량。GO주석결과표명，해가족성원작위전록인자，재식물적생장발육、역경향응등과정중발휘작용。본연구위연초급기타식물중SBP전록인자가족기인적감정화공능연구제공료기출。
The members of the SBP transcription factor family have been reported to play significant roles in regulating flower and fruit development as well as other biological processes. In this study, 32 SBP transcription factor family members were identified in Nicotiana tabacum with HMMER. Both Maximum-Likelihood phylogenetic tree and neighbor-joining tree were constructed and were found to possess similar topologies using the protein sequences of the SBP-domain. Results of phylogenetic analysis revealed that the SBP transcription factor family members could be classified into 8 subfamilies. The patterns of exon-intron structure and conserved domains in Arabidopsis and tobacco were consistent with the phylogenetic results. Transcriptome analysis showed that the expression patterns ofNtSPLs were different in different tissue types and the expression level of NtSPLsin flower was found to be significantly higher than the other tissues. GO analysis suggested that as transcription factors, the SBP family members could be involved in a series of biological processes such as developmental regulation and defense response. The results of this study provide insight into the evolution of the SBP transcription factor family inNicotiana tabacumand provide useful information for future research on these genes.