环球中医药
環毬中醫藥
배구중의약
GLOBAL TCM
2015年
9期
1089-1092
,共4页
单文超%冯会宾%赵琰%曾文浩%贺娜娜%赵妍%孙晔%屈会化
單文超%馮會賓%趙琰%曾文浩%賀娜娜%趙妍%孫曄%屈會化
단문초%풍회빈%조염%증문호%하나나%조연%손엽%굴회화
间接竞争酶联免疫分析法%葛根素%葛根%含量测定
間接競爭酶聯免疫分析法%葛根素%葛根%含量測定
간접경쟁매련면역분석법%갈근소%갈근%함량측정
ic-ELISA%Puerarin%Puerariae lobatae radix%Content determination
目的:利用间接竞争酶联免疫分析法( indirect competitive enzyme linked immunosorbent assay, ic-ELISA)测定葛根中葛根素的含量。方法利用抗葛根素单克隆抗体,通过线性关系、精密度、回收率的考察,建立葛根素的ic-ELISA分析方法。结果该方法的线性范围为15.6~500 ng/mL,孔间差和板间差均不大于3.5%,平均回收率为101.8%,并且该法检测结果与高效液相色谱法检测结果一致。结论本实验为葛根质量控制以及含葛根药材的中药复方中葛根素的含量测定提供了一种新技术方法。
目的:利用間接競爭酶聯免疫分析法( indirect competitive enzyme linked immunosorbent assay, ic-ELISA)測定葛根中葛根素的含量。方法利用抗葛根素單剋隆抗體,通過線性關繫、精密度、迴收率的攷察,建立葛根素的ic-ELISA分析方法。結果該方法的線性範圍為15.6~500 ng/mL,孔間差和闆間差均不大于3.5%,平均迴收率為101.8%,併且該法檢測結果與高效液相色譜法檢測結果一緻。結論本實驗為葛根質量控製以及含葛根藥材的中藥複方中葛根素的含量測定提供瞭一種新技術方法。
목적:이용간접경쟁매련면역분석법( indirect competitive enzyme linked immunosorbent assay, ic-ELISA)측정갈근중갈근소적함량。방법이용항갈근소단극륭항체,통과선성관계、정밀도、회수솔적고찰,건립갈근소적ic-ELISA분석방법。결과해방법적선성범위위15.6~500 ng/mL,공간차화판간차균불대우3.5%,평균회수솔위101.8%,병차해법검측결과여고효액상색보법검측결과일치。결론본실험위갈근질량공제이급함갈근약재적중약복방중갈근소적함량측정제공료일충신기술방법。
Objective To detect the content of puerarin in puerariae lobatae radix by ic-ELISA. Methods Anti-Puerarin monoclonal antibodies were used to establish ic-ELISA method for puerarin by in-vestigating the linear relationship, the precision and the recovery. Results The linear range of this method was 15. 6 ng/mL~500 ng/mL, the coefficients of variation for the intra-assay and the inter-assay are both less than 3. 5% and the average recovery was 101. 8%. The detection result of ic-ELISA method was consistent with that of HPLC test. Conclusions The ic-ELISA method can be applied to the quality control of puerariae lobatae radix and the content determination of puerarin in Chinese herbal formula.