CAJ | 학술논문

釉基质蛋白（EMP）可促进牙周膜细胞（PDLC）的增殖和多向分化能力。在EMP的作用下，PDLC中与合成细胞外基质功能相关的基因表达提高，PDLC的矿化和碱性磷酸酶（AKP）活性提高。EMP可促进骨髓基质干细胞（BMSC）成骨向分化，上调其成骨相关基因的表达，BMSC中的AKP活性提高。EMP通过上调连接蛋白-43和神经钙黏着蛋白等细胞间交流黏附因子增强成骨细胞分化。EMP可促进未成熟的成骨细胞聚集，这种聚集对连接蛋白-43和神经钙黏着蛋白上调有重要的作用，进一步提高了成骨细胞的分化和矿化活性。EMP可促进牙骨质形成，促进上皮细胞的增殖、活性和迁移。明确EMP的作用机制并利用机制调控产物，可为牙周组织缺损的临床治疗提供新的依据。
유기질단백（EMP）가촉진아주막세포（PDLC）적증식화다향분화능력。재EMP적작용하，PDLC중여합성세포외기질공능상관적기인표체제고，PDLC적광화화감성린산매（AKP）활성제고。EMP가촉진골수기질간세포（BMSC）성골향분화，상조기성골상관기인적표체，BMSC중적AKP활성제고。EMP통과상조련접단백-43화신경개점착단백등세포간교류점부인자증강성골세포분화。EMP가촉진미성숙적성골세포취집，저충취집대련접단백-43화신경개점착단백상조유중요적작용，진일보제고료성골세포적분화화광화활성。EMP가촉진아골질형성，촉진상피세포적증식、활성화천이。명학EMP적작용궤제병이용궤제조공산물，가위아주조직결손적림상치료제공신적의거。
The?proliferation?and?multi-directional?differentiation?ability?of?periodontal?ligament?cells(PDLC)?can?be?promoted?by?enamel?matrix?proteins(EMP)?and?by?the?expression?of?genes?related?to?the?synthesis?of?extracellular?matrix,?mineralization?of?PDLC,?and?the?activity?of?alkaline?phosphatase(AKP).?Similarly,?the?osteogenic?differentiation,?up?regulation?of?the?gene?expression,?and?AKP?activity?of?bone?marrow?mesenchymal?stem?cells?can?also?be?improved.?Adhesion?factors,?such?as?connexin-43?and?neural?cadherin,?could?be?upregulated?by?EMP?to?enhance?the?differentiation?of?osteoblasts.?In?addition,?the?aggregation?of?immature?osteoblasts?can?be?promoted?by?EMP,?which?plays?an?important?role?in?the?upregulation?of?connexin-43?and?neural?cadherin,?and?further?increases?the?differentiation?and?mineralization?of?osteoblasts.?Moreover,?the?formation?of?cementum?and?proliferation,?activity,?and?migration?of?epithelial?cells?can?also?be?improved?by?EMP.?Results?provide?new?theoretical?basis?for?the?clinical?treatment?of?periodontal?tissue?defectsby?discovering?the?mechanisms?underlying?EMP’s?activity?and?the?regulation?of?its?products.