CAJ | 학술논문

目的：研究梓醇对缺血细胞的保护作用机制。方法通过连二亚硫酸钠消除培养基中的氧,在37℃、5% CO2培养箱中,模拟缺血环境中的细胞损伤；再对对照组、模型组、梓醇低、中、高浓度组分别进行细胞存活率、总抗氧化能力(total antioxidant capacity,TAC)、超氧化物歧化酶( superoxide dismutase,SOD)、乳酸脱氢酶( lactate dehydrogenase,LDH)测定。结果梓醇能显著提高细胞存活率,且在5~20μmol/L呈剂量依赖性,与模型组比较,差异有统计学意义( P<0.01)；梓醇能显著提高细胞培养液上清液中的TAC、SOD,降低LDH水平,且在5~20μmol/L呈剂量依赖性,与模型组比较,P<0.01,有统计意义。结论梓醇可以通过抗氧化损伤保护缺糖缺氧细胞。
목적：연구재순대결혈세포적보호작용궤제。방법통과련이아류산납소제배양기중적양,재37℃、5% CO2배양상중,모의결혈배경중적세포손상；재대대조조、모형조、재순저、중、고농도조분별진행세포존활솔、총항양화능력(total antioxidant capacity,TAC)、초양화물기화매( superoxide dismutase,SOD)、유산탈경매( lactate dehydrogenase,LDH)측정。결과재순능현저제고세포존활솔,차재5~20μmol/L정제량의뢰성,여모형조비교,차이유통계학의의( P<0.01)；재순능현저제고세포배양액상청액중적TAC、SOD,강저LDH수평,차재5~20μmol/L정제량의뢰성,여모형조비교,P<0.01,유통계의의。결론재순가이통과항양화손상보호결당결양세포。
Objective To study the protective mechanism of catalpol onchemic cells. Methods By sodium dithionite and eliminate medium oxygen at 37℃, 5% CO2 incubator, cell damage of simulated ischemia environment. The cell survival rate, total antioxidant capacity ( totalantioxidant capacity, TAC) , superoxide dismutase ( superoxide dismutase SOD ) and lactate dehydrogenase ( lactate, dehydrogenase, LDH) determination were compared among the control group, model group, low catalpol group ,medium catalpol group and high catalpol group. Results Compared with model group, catalpol can significantly improve the survival rate of cells in a dose-dependent manner ( 5 ~20 mol/L, P<0. 01 ) . Compared with the model group, catalpol can significantly improve the cell culture supernatant of TAC, SOD, reduce the LDH level with the same trend of survival rate of cells. Conclusion Catalpol can protect OGD cell oxidative damage.