CAJ | 학술논문

丝心蛋白（SF）具有良好的生物相容性、降解性、低或无炎症反应和来源方便等特点，被广泛用作组织工程支架材料，但其力学性能差、性质不稳定，需要对其支架进行表面处理以稳定其性能，促进细胞与支架的黏附。目前，有关SF支架处理多集中在支架表面消毒、细胞与支架之间的相互作用、支架孔径的确立、支架的表面修饰等方面。SF支架消毒主要有甲醇或乙醇浸泡，紫外线、伽马射线照射等，而甲醇的毒性和挥发性会影响细胞的存活，伽马射线设备来源和紫外线的穿透能力有限，因此乙醇浸泡成为最常用的SF支架消毒方法。细胞间相互作用，SF支架孔径和微环境，SF支架表面修饰等均可增强细胞的黏附和增殖。
사심단백（SF）구유량호적생물상용성、강해성、저혹무염증반응화래원방편등특점，피엄범용작조직공정지가재료，단기역학성능차、성질불은정，수요대기지가진행표면처리이은정기성능，촉진세포여지가적점부。목전，유관SF지가처리다집중재지가표면소독、세포여지가지간적상호작용、지가공경적학립、지가적표면수식등방면。SF지가소독주요유갑순혹을순침포，자외선、가마사선조사등，이갑순적독성화휘발성회영향세포적존활，가마사선설비래원화자외선적천투능력유한，인차을순침포성위최상용적SF지가소독방법。세포간상호작용，SF지가공경화미배경，SF지가표면수식등균가증강세포적점부화증식。
Silk?fibroin(SF)?is?a?popular?tissue?engineering?material?because?of?its?good?biocompatibility,?biodegradability,?and?low?or?no?local?inflammatory?response.?However,?surface?treatment?is?still?needed?to?promote?the?adhesion?of?cells?and?scaffolds?because?SF?has?poor?mechanical?properties?and?is?unstable.?At?present,?surface?treatments?focus?on?surface?sterilization,?the?interaction?between?cells?and?scaffolds,?scaffold?diameter,?and?surface?modification?of?the?SF?scaffold.?Sterilization?methods?mainly?include?methanol?or?ethanol?immersion?disinfection?and?ultraviolet?and?gamma?ray?irradiation.?The?toxicity?and?volatility?of?methanol?affect?cell?survival,?and?gamma?ray?source?and?ultraviolet?penetration?abilities?are?limited.?Hence,?ethanol?immersion?is?the?most?commonly?used?sterilization?method.?Other?factors?that?can?enhance?the?adhesion?and?proliferation?of?cells?are?interaction?between?cells,?diameter?and?microenvironment,?and?surface?modification?of?SF.