CAJ | 학술논문

目的：研究葛根芩连汤对大鼠NASH模型的药效学作用。方法随机将SD大鼠分为空白组、模型组、葛根芩连汤低剂量组、葛根芩连汤高剂量组，每组10只，然后建立大鼠NASH模型，造模同时进行药物干预；实验第8周结束后进行血清ALT、AST、TG、TC、HDL-C、LDL-C、TNF-α、IL-6、IL-10及肝组织TNF-α、IL-6、IL-10的测定。结果（1）肝脏酶学：血清ALT、AST水平，模型组高于空白组（P＜0.01），葛根芩连汤高、低剂量组低于模型组（P＜0.05）。（2）血脂及肝脂：与空白组相比，模型组血清HDL-C显著降低，血清LDL-C、TG、TC明显增加（P＜0.01）。葛根芩连汤高、低剂量组血清HDL-C水平与模型组相比增加，血清LDL-C、TG、TC均降低（P＜0.05）。（3）血清及肝组织IL-6、IL-10、TNF-α水平：与空白组相比，模型组血清及肝组织IL-10显著降低，血清IL-6、TNF-α明显增加（P＜0.01）。与模型组相比，葛根芩连汤高、低剂量组可明显增加血清及肝组织IL-10含量，降低血清及肝组织IL-6、TNF-α水平（P＜0.05）。结论葛根芩连汤高、低剂量均能够降低高脂饮食饲喂诱导的NASH大鼠血清转氨酶水平、改善血脂代谢紊乱；能够通过提高抑炎因子和抑制促炎因子两方面调控炎症的发展。
목적：연구갈근금련탕대대서NASH모형적약효학작용。방법수궤장SD대서분위공백조、모형조、갈근금련탕저제량조、갈근금련탕고제량조，매조10지，연후건립대서NASH모형，조모동시진행약물간예；실험제8주결속후진행혈청ALT、AST、TG、TC、HDL-C、LDL-C、TNF-α、IL-6、IL-10급간조직TNF-α、IL-6、IL-10적측정。결과（1）간장매학：혈청ALT、AST수평，모형조고우공백조（P＜0.01），갈근금련탕고、저제량조저우모형조（P＜0.05）。（2）혈지급간지：여공백조상비，모형조혈청HDL-C현저강저，혈청LDL-C、TG、TC명현증가（P＜0.01）。갈근금련탕고、저제량조혈청HDL-C수평여모형조상비증가，혈청LDL-C、TG、TC균강저（P＜0.05）。（3）혈청급간조직IL-6、IL-10、TNF-α수평：여공백조상비，모형조혈청급간조직IL-10현저강저，혈청IL-6、TNF-α명현증가（P＜0.01）。여모형조상비，갈근금련탕고、저제량조가명현증가혈청급간조직IL-10함량，강저혈청급간조직IL-6、TNF-α수평（P＜0.05）。결론갈근금련탕고、저제량균능구강저고지음식사위유도적NASH대서혈청전안매수평、개선혈지대사문란；능구통과제고억염인자화억제촉염인자량방면조공염증적발전。
Objective To study the pharmacodynamic effects of Puerariae and Scutellariae and Coptidis Decoction in rats NASH model. Methods To randomly divide the SD rats with blank group, model group, Puerariae and Scutellariae and Coptidis Decoction low dose group, Puerariae and Scutellariae and Coptidis Decoction high dose group, with 10 rats in each group, then to establish rats NASH model, to build rats model meanwhile to carry out drug intervention. 8th weeks after completion of experiment, to mensurate the serum ALT, AST, TG, TC, HDL-C, LDL-C, TNF-α, IL-6, IL-10, and the hepatic tissue TNF-α, IL-6, IL-10.Results (1) Liver enzymology: The level of serum ALT and AST in model group were higher than which in blank group(P<0.01), while which in Puerariae and Scutellariae and Coptidis Decoction high dose group were lower than which in Puerariae and Scutellariae and Coptidis Decoction low dose group(P<0.05). (2) Blood lipid and liver lipid: Compared with blank group, the level of serum HDL-C was significantly lower in model group, and the serum LDL-C, TG, TC were obviously increased in model group(P<0.01). Compared with model group, the level of serum HDL-C in Puerariae and Scutellariae and Coptidis Decoction high and low dose group were obviously increased, the serum LDL-C, TG, TC in Puerariae and Scutellariae and Coptidis Decoction high and low dose group were obviously reduced(P<0.05). (3) Serum and hepatic tissue IL-6, IL-10, TNF-α level: Compared with blank group, serum and hepatic tissue IL-10 in model group was significantly decreased, serum IL-6, TNF-α in model group were obviously increased(P<0.01). Compared with model group, serum and hepatic tissue IL-10 in Puerariae and Scutellariae and Coptidis Decoction high and low dose group was obviously increased, while serum and hepatic tissue IL-6, TNF-α were lower(P<0.05).ConclusionPuerariae and Scutellariae and Coptidis Decoction high and low dose all could reduce the level of serum transaminase in NASH rats which was induced by the feeding of high fat diet, could improve the metabolism disorder of lipid, could regulate the development of inflammation through to improve anti-inflammatory cytokine and to inhibit promotion inflammation cytokine.