中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2015年
15期
9-11,50
,共4页
李舜法%黄建锋%曾莹%焦紫南%崔炳权
李舜法%黃建鋒%曾瑩%焦紫南%崔炳權
리순법%황건봉%증형%초자남%최병권
Preproghrelin基因%单核苷酸多态性%2型糖尿病%聚合酶链反应-限制性片段长度多态分析技术
Preproghrelin基因%單覈苷痠多態性%2型糖尿病%聚閤酶鏈反應-限製性片段長度多態分析技術
Preproghrelin기인%단핵감산다태성%2형당뇨병%취합매련반응-한제성편단장도다태분석기술
Preproghrelin gene%Single nucleotide polymorphism(SNP)%Type 2 diabetes mellitus%Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)
目的：探讨广州番禺区汉族人群2型糖尿病与Preproghrelin基因rs96217位点单核苷酸多态性的关系。方法提取番禺汉族人群中非糖尿病的正常人和2型糖尿病患者的DNA，采用PCR-RFLP，分析对照组和T2DM组Preproghrelin基因rs96217位点的基因型及组间基因型、等位基因频率分布的差异。结果对照组116例样本中，CC 96例、AC 20例， AA 0例；基因型频率分别为：82.8%、17.2%、0。T2DM组118例样本中， CC 89例、AC 24例、AA 5例，基因型频率分别为：75.4%、20.3%、4.3%。组间基因型和等位基因频率差异无统计学意义。结论广州番禺区汉族人群2型糖尿病与Preproghrelin基因rs96217位点单核苷酸多态性之间未见显著相关性。但T2DM组中发现AA基因型，而对照组未显，原因有待进一步研究。
目的：探討廣州番禺區漢族人群2型糖尿病與Preproghrelin基因rs96217位點單覈苷痠多態性的關繫。方法提取番禺漢族人群中非糖尿病的正常人和2型糖尿病患者的DNA，採用PCR-RFLP，分析對照組和T2DM組Preproghrelin基因rs96217位點的基因型及組間基因型、等位基因頻率分佈的差異。結果對照組116例樣本中，CC 96例、AC 20例， AA 0例；基因型頻率分彆為：82.8%、17.2%、0。T2DM組118例樣本中， CC 89例、AC 24例、AA 5例，基因型頻率分彆為：75.4%、20.3%、4.3%。組間基因型和等位基因頻率差異無統計學意義。結論廣州番禺區漢族人群2型糖尿病與Preproghrelin基因rs96217位點單覈苷痠多態性之間未見顯著相關性。但T2DM組中髮現AA基因型，而對照組未顯，原因有待進一步研究。
목적：탐토엄주번우구한족인군2형당뇨병여Preproghrelin기인rs96217위점단핵감산다태성적관계。방법제취번우한족인군중비당뇨병적정상인화2형당뇨병환자적DNA，채용PCR-RFLP，분석대조조화T2DM조Preproghrelin기인rs96217위점적기인형급조간기인형、등위기인빈솔분포적차이。결과대조조116례양본중，CC 96례、AC 20례， AA 0례；기인형빈솔분별위：82.8%、17.2%、0。T2DM조118례양본중， CC 89례、AC 24례、AA 5례，기인형빈솔분별위：75.4%、20.3%、4.3%。조간기인형화등위기인빈솔차이무통계학의의。결론엄주번우구한족인군2형당뇨병여Preproghrelin기인rs96217위점단핵감산다태성지간미견현저상관성。단T2DM조중발현AA기인형，이대조조미현，원인유대진일보연구。
Objective To study the relationships between type 2 diabetes mellitus (T2DM) in Han ethnics of Panyu and SNP rs96217 of preproghrelin gene.Methods Extraction DNA of 116 non-T2DM healthy individuals (the control group) and 118 T2DM individuals (the T2DM group) from populations of Han ethnics in Panyu, target gene segments were detected using PCR-RFLP in order to analyze genotypes of SNP rs96217 of preproghrelin gene, and to compare the differences of genotypic and allelic frequencies between the two groups.Results In the control group, the genotypes CC and AC had 96 and 20 individuals, but genotype AA wasn’t find. Their genotypic frequencies were 82.8%,17.2% and 0%, respectivel. In the T2DM group, the genotypes CC, AC and AA were detected in 89, 24 and 5 cases, Their genotypic frequencies were 75.4%、20.3% and 4.3%, respectively. there were no statistical significance in the difference of genotypic and allelic frequencies between the control group and the T2DM group.Conclusion There are no significant relationships between T2DM in Han ethnics of Panyu and SNP rs96217 of preproghrelin gene. However, genotype AA was detected in the T2DM group but not in control, which suggested that allele A may play certain roles in T2DM development. Further investigations certainly would be worth pursuing.
