CAJ | 학술논문

目的：探讨肺癌细胞中SOX1基因是否直接调控ECT2的表达。方法运用甲基化特异PCR法检测50例原发肺癌和癌旁组织中SOX1的甲基化水平。以肺癌细胞株A-1细胞为研究对象，Realtime-PCR检测ECT2表达情况。通过荧光素酶报告检查法以及染色体免疫共沉淀方法检测SOX1基因是否直接调控ECT2的表达。免疫组化检测50例肺癌组织中ECT2与SOX1表达的关系。结果原发性肺癌的甲基化异常检出率是70%（35/50）。过表达SOX1后A-1细胞中ECT2表达下降，而siRNA抑制SOX1后ECT2表达升高，荧光素酶报告检查法以及染色体免疫共沉淀方法提示SOX1可直接与ECT2启动子结合，抑制ECT2的表达。SOX1与ECT2在肺癌组织中表达负相关（r=-0.433，P=0.001）。结论肺癌细胞A-1中SOX1基因直接调控ECT2的表达。
목적：탐토폐암세포중SOX1기인시부직접조공ECT2적표체。방법운용갑기화특이PCR법검측50례원발폐암화암방조직중SOX1적갑기화수평。이폐암세포주A-1세포위연구대상，Realtime-PCR검측ECT2표체정황。통과형광소매보고검사법이급염색체면역공침정방법검측SOX1기인시부직접조공ECT2적표체。면역조화검측50례폐암조직중ECT2여SOX1표체적관계。결과원발성폐암적갑기화이상검출솔시70%（35/50）。과표체SOX1후A-1세포중ECT2표체하강，이siRNA억제SOX1후ECT2표체승고，형광소매보고검사법이급염색체면역공침정방법제시SOX1가직접여ECT2계동자결합，억제ECT2적표체。SOX1여ECT2재폐암조직중표체부상관（r=-0.433，P=0.001）。결론폐암세포A-1중SOX1기인직접조공ECT2적표체。
Objective To test whether SOX1 is a novel transcriptional repressor for ECT2 in human lung cancer. Methods 50 primary lung cancer tissues and corresponding normal tissues were detected by Methylation-specific PCR method.The expression of ECT2 in the A-1 cell was detected by Realtime-PCR.We determined whether SOX1 may regulate the expression of ECT2 by using Chromin immunoprecipitation (ChIP) assay and Luciferase activity assay.Immunohistochemical analysis was used to test whether there is an inverse correlation between ECT2 and SOX1. Results Aberrant methylation in primary lung cancer was 70% (35/50).Ectopic expression of SOX1 in the A-1 cell repressed ECT2 expression.Conversely,siRNA silencing of the SOX1 gene increased ECT2 expression.We also show that SOX1 directly interacted with and repressed the ECT2 promoter.Moreover,the analysis of 50 primary lung cancer samples revealed an inverse correlation between ECT2 and SOX1 levels(r=-0.433,P=0.001).ConclusionSOX1 is a novel transcriptional repressor for ECT2 in human lung cancer.