胃肠病学
胃腸病學
위장병학
CHINESE JOURNAL OF GASTROENTEROLOGY
2015年
8期
457-461
,共5页
张涛%张敏%汤爱荣%曹萍%谢丽娟%于成功
張濤%張敏%湯愛榮%曹萍%謝麗娟%于成功
장도%장민%탕애영%조평%사려연%우성공
Faecalibacterium prausnitzii%干扰素 γ%白细胞介素 4%Th1-Th2 平衡%结肠肿瘤%LoVo 细胞
Faecalibacterium prausnitzii%榦擾素 γ%白細胞介素 4%Th1-Th2 平衡%結腸腫瘤%LoVo 細胞
Faecalibacterium prausnitzii%간우소 γ%백세포개소 4%Th1-Th2 평형%결장종류%LoVo 세포
Faecalibacterium prausnitzii%Interferon-gamma%Interleukin-4%Th1-Th2 Balance%Colonic Neoplasms%LoVo Cells
Background:Faecalibacterium prausnitzii(Fp)is a commensal intestinal bacterium that exhibits anti-inflammatory and immunomodulatory capacity in vivo and in vitro. It has been reported that Fp in intestinal lumen was reduced in patients with colorectal cancer,which might be a factor associated with cancer development. Aims:To investigate the effect and immunological mechanism of Fp and its genomic DNA(fDNA)on the killing activity of peripheral blood mononuclear cells (PBMCs)against human colon cancer LoVo cells. Methods:PBMCs derived from healthy adults were co-cultured in vitro with Fp,fDNA,or the digested fDNA(d-fDNA),respectively. Killing activity of PBMCs against LoVo cells was measured by MTT assay;concentrations of interferon-gamma(INF-γ),a Th1-type cytokine and interleukin-4(IL-4),a Th2-type cytokine in culture supernatant of PBMCs were determined by ELISA;and expressions of T-bet and GATA3,the transcription factors specific for Th1 and Th2 cells,were measured by real-time PCR. Results:Compared with the PBMCs not treated,fDNA could significantly enhance the killing activity of PBMCs against LoVo cells(P < 0. 05);meanwhile,it promoted IFN-γ secretion,up-regulated T-bet mRNA expression and inhibited IL-4 secretion and GATA3 mRNA expression in PBMCs(P < 0. 05). Similar effects were not observed in PBMCs treated with Fp and d-fDNA. Conclusions:fDNA enhances the killing activity of PBMCs against human colon cancer cells by up-regulating Th1 immune response.