中国执业药师
中國執業藥師
중국집업약사
CHINA LICENSED PHARMACIST
2015年
9期
36-39
,共4页
赵群涛%杨俊杰%方永凯%盚罡%李丹
趙群濤%楊俊傑%方永凱%盚罡%李丹
조군도%양준걸%방영개%구강%리단
归脾丸(浓缩丸)%酸枣仁皂苷A%黄芪甲苷%高效液相色谱紫外检测法
歸脾汍(濃縮汍)%痠棘仁皂苷A%黃芪甲苷%高效液相色譜紫外檢測法
귀비환(농축환)%산조인조감A%황기갑감%고효액상색보자외검측법
Guipi Pills ( Concentrated Pills )%Jujuboside A%Astragaloside Ⅳ%HPLC-UV
目的：建立高效液相色谱紫外检测法（HPLC-UV）同时测定归脾丸（浓缩丸）中酸枣仁皂苷A和黄芪甲苷含量的方法。方法：色谱柱为 Thermo Hypersil BDS C18（250 mm ×4.6 mm ，5μm）；以乙腈-水为流动相（28∶72），检测波长204 nm；流速1.0 mL/min；柱温28℃。结果：酸枣仁皂苷A进样量在2.53～15.18μg ，黄芪甲苷进样量在2.55～15.30μg范围内呈良好线性关系，回收率分别为99.1%和98.4%。结论：该方法灵敏、重复性好，可用于归脾丸（浓缩丸）的质量控制。
目的：建立高效液相色譜紫外檢測法（HPLC-UV）同時測定歸脾汍（濃縮汍）中痠棘仁皂苷A和黃芪甲苷含量的方法。方法：色譜柱為 Thermo Hypersil BDS C18（250 mm ×4.6 mm ，5μm）；以乙腈-水為流動相（28∶72），檢測波長204 nm；流速1.0 mL/min；柱溫28℃。結果：痠棘仁皂苷A進樣量在2.53～15.18μg ，黃芪甲苷進樣量在2.55～15.30μg範圍內呈良好線性關繫，迴收率分彆為99.1%和98.4%。結論：該方法靈敏、重複性好，可用于歸脾汍（濃縮汍）的質量控製。
목적：건립고효액상색보자외검측법（HPLC-UV）동시측정귀비환（농축환）중산조인조감A화황기갑감함량적방법。방법：색보주위 Thermo Hypersil BDS C18（250 mm ×4.6 mm ，5μm）；이을정-수위류동상（28∶72），검측파장204 nm；류속1.0 mL/min；주온28℃。결과：산조인조감A진양량재2.53～15.18μg ，황기갑감진양량재2.55～15.30μg범위내정량호선성관계，회수솔분별위99.1%화98.4%。결론：해방법령민、중복성호，가용우귀비환（농축환）적질량공제。
Objective: To establish a method for the simultaneous determination of the contents of jujuboside A and astragaloside Ⅳ in guipi pills ( concentrated pills ) by HPLC-UV . Methods: HPLC-UV was performed on Thermo Hypersil BDS C18 column (250 mm × 4.6 mm,5 μm) with a mobile phase of acetonitrile-water(28∶72), detection wavelength was at 204 nm , the flow rate was 1 . 0 mL/min and the column temperature was at 28 ℃. Results: A good linear relationship was obtained within the range of 2 . 53 ~ 15 . 18 μg and 2 . 55 ~ 15 . 30 μg for jujuboside A and astragaloside Ⅳ, and the recovery rate was 99 . 1% and 98 . 4% respectively . Conclusion: The method was sensitive and reproducible and is suitable for the quality control of guipi pills ( concentrated pills ) .
