CAJ | 학술논문

目的：观察槲皮素-3-葡萄糖苷对急性肾缺血－再灌注损伤大鼠的保护作用。方法：采用夹闭大鼠双侧肾动脉45min后再灌注60min的方法，建立大鼠急性肾缺血－再灌注损伤的动物模型。通过测定血清丙二醛（MDA）、超氧化物歧化酶（SOD）、血清肌酐（Cr）、尿素氮（BUN），和TUNEL法分析肾小管上皮细胞的凋亡情况，探讨槲皮素-3-葡萄糖苷对急性肾缺血－再灌注损伤大鼠的保护作用及可能机制。结果：槲皮素-3-葡萄糖苷组MDA值显著降低（P<0.05）；血清SOD含量升高明显；血清NO含量较模型组明显下降，两组比较有显著性差异（P<0.05）。槲皮素-3-葡萄糖苷组的 TUNEL 阳性细胞数与模型组相比明显减少（P<0.05）。结论：槲皮素-3-葡萄糖苷可以减轻肾小管细胞的损伤，对肾缺血－再灌注损伤大鼠具有保护作用，其作用机制可能是通过清除氧自由基，抑制脂质过氧化，进而改善肾功能。
목적：관찰곡피소-3-포도당감대급성신결혈－재관주손상대서적보호작용。방법：채용협폐대서쌍측신동맥45min후재관주60min적방법，건립대서급성신결혈－재관주손상적동물모형。통과측정혈청병이철（MDA）、초양화물기화매（SOD）、혈청기항（Cr）、뇨소담（BUN），화TUNEL법분석신소관상피세포적조망정황，탐토곡피소-3-포도당감대급성신결혈－재관주손상대서적보호작용급가능궤제。결과：곡피소-3-포도당감조MDA치현저강저（P<0.05）；혈청SOD함량승고명현；혈청NO함량교모형조명현하강，량조비교유현저성차이（P<0.05）。곡피소-3-포도당감조적 TUNEL 양성세포수여모형조상비명현감소（P<0.05）。결론：곡피소-3-포도당감가이감경신소관세포적손상，대신결혈－재관주손상대서구유보호작용，기작용궤제가능시통과청제양자유기，억제지질과양화，진이개선신공능。
Objective: To observe protective effects of quercetin -3- grape glycosides on acute renal ischemia reperfusion injury in rats. Methods: A rat model of acute renal ischemia reperfusion injury was established by the 60min method after the 45min of the bilateral renal artery. Through the determination of serum malondialdehyde (MDA), superoxide dismutase (SOD), serum creatinine (CR), blood urea nitrogen (BUN), and TUNEL assay of apoptosis of renal tubular epithelial cells and the protection and possible mechanism of Quercitrin-3-glucoside on acute renal ischemia - reperfusion injury in rats were investigated. Results: In Quercitrin-3-glucoside group, MDA decreased significantly (P<0.05); serum SOD content increased significantly; compared with the model group, the content of serum NO decreased significantly, with significant difference (P<0.05). The number of TUNEL positive cells was significantly decreased (P<0.05). Conclusion: Quercitrin-3-glucoside can alleviate the injury of renal tubular cells, has a protective effect on renal ischemia - reperfusion injury in rats. The mechanism may improve renal function by scavenging oxygen free radicals and inhibiting lipid peroxidation.