浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2015年
8期
615-620
,共6页
快速高分离液相色谱-质谱法%减肥药%盐酸西布曲明%呋塞米%盐酸麻黄碱%含量
快速高分離液相色譜-質譜法%減肥藥%鹽痠西佈麯明%呋塞米%鹽痠痳黃堿%含量
쾌속고분리액상색보-질보법%감비약%염산서포곡명%부새미%염산마황감%함량
RRLC-MS%diet pills%sibutramine hydrochloride%furosemide%ephedrine hydrochloride%content
[目的]建立盐酸西布曲明、呋塞米和盐酸麻黄碱的快速高分离液相色谱-质谱联用（rapid resolution liquid chromatography-mass spectrometry, RRLC-MS）方法，检测7种减肥药中是否含有非法添加成分。[方法]色谱条件：Agilent SB-C18柱（2.1mm×50mm,5μm），乙腈（含0.1%甲酸）-0.1%甲酸水溶液为流动相进行梯度洗脱，流速0.2mL·min-1；质谱条件：电喷雾离子（electrospray ionization, ESI）源，以正负离子、多反应监测（multiple reaction monitoring, MRM）扫描模式检测减肥药中违禁成分。[结果]7种减肥药中均检测到盐酸西布曲明和盐酸麻黄碱，含量范围分别为0.39～4.29μg·g-1和1.63～8984.18μg·g-1，1种减肥药中检测到呋塞米，含量为8.71μg·g-1。[结论]本研究建立的快速高分离液相色谱-质谱联用法方便快捷、灵敏度好、准确度高，适用于减肥药中非法添加成分的定性定量检测。
[目的]建立鹽痠西佈麯明、呋塞米和鹽痠痳黃堿的快速高分離液相色譜-質譜聯用（rapid resolution liquid chromatography-mass spectrometry, RRLC-MS）方法，檢測7種減肥藥中是否含有非法添加成分。[方法]色譜條件：Agilent SB-C18柱（2.1mm×50mm,5μm），乙腈（含0.1%甲痠）-0.1%甲痠水溶液為流動相進行梯度洗脫，流速0.2mL·min-1；質譜條件：電噴霧離子（electrospray ionization, ESI）源，以正負離子、多反應鑑測（multiple reaction monitoring, MRM）掃描模式檢測減肥藥中違禁成分。[結果]7種減肥藥中均檢測到鹽痠西佈麯明和鹽痠痳黃堿，含量範圍分彆為0.39～4.29μg·g-1和1.63～8984.18μg·g-1，1種減肥藥中檢測到呋塞米，含量為8.71μg·g-1。[結論]本研究建立的快速高分離液相色譜-質譜聯用法方便快捷、靈敏度好、準確度高，適用于減肥藥中非法添加成分的定性定量檢測。
[목적]건립염산서포곡명、부새미화염산마황감적쾌속고분리액상색보-질보련용（rapid resolution liquid chromatography-mass spectrometry, RRLC-MS）방법，검측7충감비약중시부함유비법첨가성분。[방법]색보조건：Agilent SB-C18주（2.1mm×50mm,5μm），을정（함0.1%갑산）-0.1%갑산수용액위류동상진행제도세탈，류속0.2mL·min-1；질보조건：전분무리자（electrospray ionization, ESI）원，이정부리자、다반응감측（multiple reaction monitoring, MRM）소묘모식검측감비약중위금성분。[결과]7충감비약중균검측도염산서포곡명화염산마황감，함량범위분별위0.39～4.29μg·g-1화1.63～8984.18μg·g-1，1충감비약중검측도부새미，함량위8.71μg·g-1。[결론]본연구건립적쾌속고분리액상색보-질보련용법방편쾌첩、령민도호、준학도고，괄용우감비약중비법첨가성분적정성정량검측。
Objective]To establish a rapid resolution liquid chromatography-mass spectrometry(RRLC-MS) method for sibutramine hydrochloride, ephedrine hydrochloride and furosemide, which is the illegally added substances in diet pills. [Method]The chromatographic conditions: Agilent SB-C18 column(2.1mm×50mm, 5μm). The mobile phase consists of acetonitrile(containing 0.1% formic acid) and 0.1% formic acid solution, gradient elution with flow rate of 0.2mL·min-1; The MS conditions: electrospray ionization(ESI) source, with positive and negative ions, multiple reaction monitoring(MRM) mode to detect contents of prohibited substances in diet pills. [Result]Under the above conditions, all seven diet pills were detected with the sibutramine hydrochloride and ephedrine hydrochloride. The concentration range of the two substances was from 0.39 to 4.29μg·g-1 and from 1.63 to 8984.18μg·g-1 respectively. One drug contained furosemide, while the content was 8.71μg·g-1. [Conclusion]The method established was fast, convenient, had high sensitivity and high accuracy, which can detect prohibited substances quantitatively and qualitatively in diet pills.
