CAJ | 학술논문

目的：探讨微小RNA?100（ miR?100）在结直肠癌组织中的表达情况及其与临床病理特征及预后的关系。方法采用实时定量PCR方法检测172例结直肠癌组织及癌旁组织中miR?100的表达水平，并分析其与临床病理特征及预后的关系。构建miR?100高表达结直肠癌HCT?8?miR?100细胞及对照HCT?8?NC细胞，采用四甲基偶氮唑蓝法检测细胞增殖，采用流式细胞仪检测细胞周期和凋亡情况，采用划痕实验和Transwell实验检测细胞迁移和侵袭。结果结直肠癌组织和癌旁组织中miR?100的相对表达水平分别为－6．185±1．921和－3．698±1．786，差异有统计学意义（P＜0．01）。有淋巴结转移和无淋巴结转移的结直肠癌组织中miR?100的相对表达水平分别为－5．706±1．809和－6．775±1．902，差异有统计学意义（ P＜0．01）。Ⅰ期、Ⅱ期、Ⅲ期和Ⅳ期结直肠癌组织中miR?100的相对表达水平分别为－7．267±1．888、－6．443±1．859、－5．923±1．796和－4．639±1．516，差异有统计学意义（P＜0．01）。高、中、低分化结直肠癌组织中miR?100的相对表达水平分别为－7．389±1．828、－6．095±1．843和－5．476±2．088，差异有统计学差异（ P＜0．01）。生存分析显示，miR?100高表达组和低表达组患者术后随访41个月的生存率分别为78．0％和86．0％，差异无统计学意义（P＝0．179）。细胞实验显示，miR?100高表达可抑制结直肠癌HCT?8细胞增殖，促进细胞凋亡和细胞迁移。结论 miR?100表达与结直肠癌淋巴结转移、肿瘤分化程度和TNM 分期有关。 miR?100有可能作为提示结直肠癌淋巴结转移及恶性进展的潜在标志物。
목적：탐토미소RNA?100（ miR?100）재결직장암조직중적표체정황급기여림상병리특정급예후적관계。방법채용실시정량PCR방법검측172례결직장암조직급암방조직중miR?100적표체수평，병분석기여림상병리특정급예후적관계。구건miR?100고표체결직장암HCT?8?miR?100세포급대조HCT?8?NC세포，채용사갑기우담서람법검측세포증식，채용류식세포의검측세포주기화조망정황，채용화흔실험화Transwell실험검측세포천이화침습。결과결직장암조직화암방조직중miR?100적상대표체수평분별위－6．185±1．921화－3．698±1．786，차이유통계학의의（P＜0．01）。유림파결전이화무림파결전이적결직장암조직중miR?100적상대표체수평분별위－5．706±1．809화－6．775±1．902，차이유통계학의의（ P＜0．01）。Ⅰ기、Ⅱ기、Ⅲ기화Ⅳ기결직장암조직중miR?100적상대표체수평분별위－7．267±1．888、－6．443±1．859、－5．923±1．796화－4．639±1．516，차이유통계학의의（P＜0．01）。고、중、저분화결직장암조직중miR?100적상대표체수평분별위－7．389±1．828、－6．095±1．843화－5．476±2．088，차이유통계학차이（ P＜0．01）。생존분석현시，miR?100고표체조화저표체조환자술후수방41개월적생존솔분별위78．0％화86．0％，차이무통계학의의（P＝0．179）。세포실험현시，miR?100고표체가억제결직장암HCT?8세포증식，촉진세포조망화세포천이。결론 miR?100표체여결직장암림파결전이、종류분화정도화TNM 분기유관。 miR?100유가능작위제시결직장암림파결전이급악성진전적잠재표지물。
Objective The aim of this study was to investigate the expression of microRNA?100 ( miR?100) and its relation with prognosis in colorectal cancer ( CRC) . Methods The expression of miR?100 was analyzed by quantitative real?time PCR ( qRT?PCR) in 172 CRC tissue samples. The relation of miR?100 expression patterns with clinical pathological significance in CRC was analyzed. The effects of alterations of miR?100 expression and its consequences on CRC cell proliferation, apoptosis and migration were demonstrated in cells cultured in vitro. Results The relative expression of miR?100 in CRC tissues and peritumoral tissues were -6. 185 ± 1. 921 and -3. 698 ± 1. 786, respectively, with a significant difference between the two groups( P<0.01) . There was a significant difference between the relative expression of miR?100 in CRC with lymph node metastasis (-5.706±1.809) and without lymph node metastasis (-6.775± 1.902, P<0.01). The relative expression of miR?100 in tumors of different TNM stages were -7.267±1.888 in stage Ⅰ, -6.443±1.859 in stageⅡ,-5.923±1.796 in stageⅢ, and-4.639±1.516 in stageⅣ, with a significant difference among them(P<0.01). Different differentiation grades showed different expression of miR?100, i.e. -7. 389 ± 1. 828 in well differentiated tumors, -6. 095 ± 1. 843 in moderately differentiated tumors, and -5.476±2.088 in poorly differentiated tumors (P<0.01). There was no significant correlation between miR?100 expression and overall survival rates of the CRC patients (P=0.179). Overexpression of miR?100 in the CRC cell line HCT?8 inhibited cell proliferation, but promoted cell apoptosis and migration. Conclusions The expression of miR?100 is correlated with lymph node metastasis, TNM stage and differentiation grade, and may be a potential biomarker indicating the development of CRC.