CAJ | 학술논문

目的：通过建立Balb／c小鼠光老化动物模型，探讨灯盏花素抗皮肤光老化作用机制，为光老化的防治及护肤品的研发提供理论依据。方法 Balb／c小鼠随机分为7组，模拟日光中UV（ UVA＋UVB）长期照射，建立光老化动物模型，经9周UV照射及外搽受试品后，检测各组小鼠受试部位经皮水分丢失量（ TEWL）、基质金属蛋白酶1（ MMP-1）的表达及细胞因子白介素1α（ IL-1α）和细胞因子白介素6（ IL-6）的含量。结果模型组小鼠检测的TEWL值、MMP-1表达及IL-1α、IL-6含量均明显升高（ P＜0.05）；F组、G组小鼠检测的TEWL值、MMP-1表达及IL-1α、IL-6含量均明显降低（ P＜0.05）；其中F组作用更明显。结论外用灯盏花素可有助于修复Balb／c小鼠光老化模型的皮肤屏障功能，下调炎症细胞因子分泌水平，抑制MMP-1表达，具有抗光老化作用。
목적：통과건립Balb／c소서광노화동물모형，탐토등잔화소항피부광노화작용궤제，위광노화적방치급호부품적연발제공이론의거。방법 Balb／c소서수궤분위7조，모의일광중UV（ UVA＋UVB）장기조사，건립광노화동물모형，경9주UV조사급외차수시품후，검측각조소서수시부위경피수분주실량（ TEWL）、기질금속단백매1（ MMP-1）적표체급세포인자백개소1α（ IL-1α）화세포인자백개소6（ IL-6）적함량。결과모형조소서검측적TEWL치、MMP-1표체급IL-1α、IL-6함량균명현승고（ P＜0.05）；F조、G조소서검측적TEWL치、MMP-1표체급IL-1α、IL-6함량균명현강저（ P＜0.05）；기중F조작용경명현。결론외용등잔화소가유조우수복Balb／c소서광노화모형적피부병장공능，하조염증세포인자분비수평，억제MMP-1표체，구유항광노화작용。
Objective To investigate the mechanism of anti-photoaging of Breviscapine to provides a theoretical basis for prevention of photoaging and development of skin care product.Methods Healthy Balb/c mice were divided into 7 groups randomly and irradiated by ultraviolet irradiation ( UVA and UVB) to simulate skin photoageing.After <br> nine weeks of ultraviolet irradiation and topical application of breviscapin, The value of transepidermal water loss ( TEWL) was measured, and the serum level of MMP-1, IL-1α, IL-6 in different experimental group were detec-ted.Results The TEWL value and the expression of MMP-1, IL-1αand IL-6 were significantly increased ( P<0. 05) in the blank group.The TEWL value and the expression of MMP-1, IL-1αand IL-6 were significantly de-creased in group F and group G ( P<0.05) .Conclusion Topical application of Breviscapine have an important effect on anti-photoaging, repairing of skin barrier function, which result from decreased expression of MMP-1.