北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2015年
9期
3-4,5
,共3页
金果饮%橙皮苷%质量标准%薄层%高效液相
金果飲%橙皮苷%質量標準%薄層%高效液相
금과음%등피감%질량표준%박층%고효액상
Jinguo Yin%Hesperidin%Quality control%TLC%HPLC
目的:对金果饮的质量标准进行制定。方法:对玄参、西青果、陈皮建立薄层色谱的鉴别方法;采用高效液相色谱法测定橙皮苷的含量。用Agilent SB C18柱(4.6mm×250mm,5μm),以甲醇-6%乙酸溶液(35∶65)为流动相;检测波长为283nm,流速1mL·min-1。结果:薄层色谱鉴别中,供试品在与对照药材色谱相应的位置上,显示相同颜色的斑点,斑点清晰,分离效果好,阴性无干扰;含量测定法中,橙皮苷在36.35~726.95ng(r=0.9999,n=5)范围内呈良好的线性关系,平均加样回收率为98.58%(RSD为1.85%)。结论:建立的方法简便、准确、专属性强、重复性好,能有效用于金果饮的质量控制。
目的:對金果飲的質量標準進行製定。方法:對玄參、西青果、陳皮建立薄層色譜的鑒彆方法;採用高效液相色譜法測定橙皮苷的含量。用Agilent SB C18柱(4.6mm×250mm,5μm),以甲醇-6%乙痠溶液(35∶65)為流動相;檢測波長為283nm,流速1mL·min-1。結果:薄層色譜鑒彆中,供試品在與對照藥材色譜相應的位置上,顯示相同顏色的斑點,斑點清晰,分離效果好,陰性無榦擾;含量測定法中,橙皮苷在36.35~726.95ng(r=0.9999,n=5)範圍內呈良好的線性關繫,平均加樣迴收率為98.58%(RSD為1.85%)。結論:建立的方法簡便、準確、專屬性彊、重複性好,能有效用于金果飲的質量控製。
목적:대금과음적질량표준진행제정。방법:대현삼、서청과、진피건립박층색보적감별방법;채용고효액상색보법측정등피감적함량。용Agilent SB C18주(4.6mm×250mm,5μm),이갑순-6%을산용액(35∶65)위류동상;검측파장위283nm,류속1mL·min-1。결과:박층색보감별중,공시품재여대조약재색보상응적위치상,현시상동안색적반점,반점청석,분리효과호,음성무간우;함량측정법중,등피감재36.35~726.95ng(r=0.9999,n=5)범위내정량호적선성관계,평균가양회수솔위98.58%(RSD위1.85%)。결론:건립적방법간편、준학、전속성강、중복성호,능유효용우금과음적질량공제。
Objective:To establish quality control of Jinguo Yin.Methods:TLC method was used to differentiate scrophulariae radix, terminalia chebula retz.and citri pericarpium reticulatae. HPLC method was used to determine content of hesperidin.C18 column (4.6mm× 250mm,5μm)was used with methanol and 6%acetic acid(35∶65) as a mobile phase.The flow rate was 1mL·min-1,detection wavelength was 283 nm. Results:Samples and controls had the same color fluorescence spots at the same position in the TLC method.Spots were well isolated.In the HPLC method, linear range was 36.35~726.95ng (r =0.9999, n=5).Average recovery was 98.58%(RSD=1.85%,n=9). Conclusion:The established method was sensitive,accurate,repeatable and specific.It can be used for quality control of Jinguo Yin.