山东医学高等专科学校学报
山東醫學高等專科學校學報
산동의학고등전과학교학보
JOURNAL OF SHANDONG MEDICAL COLLEGE
2015年
4期
247-250
,共4页
匹诺塞林%局灶性脑缺血再灌注%炎症%TNFα%IL-1β
匹諾塞林%跼竈性腦缺血再灌註%炎癥%TNFα%IL-1β
필낙새림%국조성뇌결혈재관주%염증%TNFα%IL-1β
Pinocembrin%Facal cerebral ischemia reperfusion%Inflammation%TNFα%IL-1β
目的：探讨匹诺塞林对局灶性脑缺血再灌注损伤大鼠炎症反应的影响，分析其治疗缺血性脑卒中的机制。方法雄性SD大鼠50只，随机分为假手术组、模型组、匹诺塞林1mg／kg ，3mg／kg ，10mg／kg治疗组，采用大脑中动脉阻塞法（middle cerebral artery occlusion ，MCAO）建立脑缺血再灌注模型，分别与大脑中动脉阻塞2h再灌注6h后，观察缺血脑组织水肿情况，计算脑水肿百分比；酶联免疫吸附测定法（enzyme linked immunosorbent assay ，ELISA ）检测大鼠血清肿瘤坏死因子α（（tumor necrosis factor α，TNFα）及白细胞介素1β（interleukin‐1β，IL‐1β）含量。结果大脑中动脉阻塞2h再灌注后6 h ，与模型组比较，匹诺塞林各治疗组脑水肿百分比降低（ P＜0．05），脑水肿明显减轻；血清T N Fα及IL‐1β含量明显降低（ P ＜0．01或 P ＜0．05）。结论匹诺塞林具有减轻大鼠脑缺血再灌注损伤炎症反应的作用。
目的：探討匹諾塞林對跼竈性腦缺血再灌註損傷大鼠炎癥反應的影響，分析其治療缺血性腦卒中的機製。方法雄性SD大鼠50隻，隨機分為假手術組、模型組、匹諾塞林1mg／kg ，3mg／kg ，10mg／kg治療組，採用大腦中動脈阻塞法（middle cerebral artery occlusion ，MCAO）建立腦缺血再灌註模型，分彆與大腦中動脈阻塞2h再灌註6h後，觀察缺血腦組織水腫情況，計算腦水腫百分比；酶聯免疫吸附測定法（enzyme linked immunosorbent assay ，ELISA ）檢測大鼠血清腫瘤壞死因子α（（tumor necrosis factor α，TNFα）及白細胞介素1β（interleukin‐1β，IL‐1β）含量。結果大腦中動脈阻塞2h再灌註後6 h ，與模型組比較，匹諾塞林各治療組腦水腫百分比降低（ P＜0．05），腦水腫明顯減輕；血清T N Fα及IL‐1β含量明顯降低（ P ＜0．01或 P ＜0．05）。結論匹諾塞林具有減輕大鼠腦缺血再灌註損傷炎癥反應的作用。
목적：탐토필낙새림대국조성뇌결혈재관주손상대서염증반응적영향，분석기치료결혈성뇌졸중적궤제。방법웅성SD대서50지，수궤분위가수술조、모형조、필낙새림1mg／kg ，3mg／kg ，10mg／kg치료조，채용대뇌중동맥조새법（middle cerebral artery occlusion ，MCAO）건립뇌결혈재관주모형，분별여대뇌중동맥조새2h재관주6h후，관찰결혈뇌조직수종정황，계산뇌수종백분비；매련면역흡부측정법（enzyme linked immunosorbent assay ，ELISA ）검측대서혈청종류배사인자α（（tumor necrosis factor α，TNFα）급백세포개소1β（interleukin‐1β，IL‐1β）함량。결과대뇌중동맥조새2h재관주후6 h ，여모형조비교，필낙새림각치료조뇌수종백분비강저（ P＜0．05），뇌수종명현감경；혈청T N Fα급IL‐1β함량명현강저（ P ＜0．01혹 P ＜0．05）。결론필낙새림구유감경대서뇌결혈재관주손상염증반응적작용。
Objective To investigate the effects of pinocembrin on inflammation in rats after focal cere‐bral ischemia reperfusion(I/R) ,and to analyze the mechanism of pinocembrin in treating ischemic stroke . Methods 50 male Sprague‐Dawley rats were randomly divided into five groups :the sham group ,the model group and three pinocembrin groups .Focal cerebral ischemia reperfusion rats were induced by middle cere‐bral artery occlusion (MCAO) for 2h followed by 6h reperfusion .Pinocembrin was administered in differ‐ent doses (1mg/kg ,3mg/kg and 10mg/kg respectively) at the same time of the onset of reperfusion ,then the percentage of cerebral edema of ischemia brain in the rats was calculated and the serum levels of TNFαand IL‐1βwere detected by enzyme linked immunosorbent assay .Results Pinocembrin (1mg/kg ,3mg/kg and 10mg/kg ) significantly reduced the percentage of cerebral edema of ischemia brain and the levels of serum TNFα and IL‐1β compared with those in the model group .Conclusion Pinocembrin may inhibit inflamma‐tory response in I/R rats ,w hich may partly account for the mechanism of treating ischemic stroke .
