CAJ | 학술논문

目的：研究Survivin拮抗肽对裸鼠脑胶质瘤的抑制效应。方法将体外培养的人胶质瘤 U251细胞经立体定向技术注入裸鼠脑尾状核区域，构建荷U 251胶质瘤裸鼠原位模型。荷瘤11 d后，随机分成实验组和对照组，分别经腹腔注射Survivin拮抗肽、生理盐水，30 d后全部处死，制作病理切片和HE染色。应用Olimpus CX41显微镜图像分析系统，计算在肿瘤组织的最大切面上，肿瘤坏死面积与肿瘤面积的比值。结果 Survivin拮抗肽实验组裸鼠的肿瘤坏死面积与肿瘤面积的比值大于对照组，差异具有统计学意义（P＜0.05）。结论 Survivin拮抗肽对人胶质瘤 U251细胞裸鼠移植瘤的生长具有抑制效应。
목적：연구Survivin길항태대라서뇌효질류적억제효응。방법장체외배양적인효질류 U251세포경입체정향기술주입라서뇌미상핵구역，구건하U 251효질류라서원위모형。하류11 d후，수궤분성실험조화대조조，분별경복강주사Survivin길항태、생리염수，30 d후전부처사，제작병리절편화HE염색。응용Olimpus CX41현미경도상분석계통，계산재종류조직적최대절면상，종류배사면적여종류면적적비치。결과 Survivin길항태실험조라서적종류배사면적여종류면적적비치대우대조조，차이구유통계학의의（P＜0.05）。결론 Survivin길항태대인효질류 U251세포라서이식류적생장구유억제효응。
Objective To investigate the inhibiting effect of Survivin anti‐peptide on nude rats with brain glioma.Methods The rat orthotopic model of U251 glioma was established through transplanting human glioma U251 cells cultured in vitro in‐to caudate nucleus of nude rats by stereotactic technique. After 11 days ,all nude rats were randomly divided into experimental group and control group ,which were separately injected Survivin anti‐peptide and normal saline via abdomen injection. After 30 days ,we executed all of nude rats and made pathological sections and HE staining. Then the ratio of tumor necrotic area and tumor total area was calculated on the largest slice of tumor tissues by the analysis system of Olimpus CX41 microscope im‐age.Results The ratio of tumor necrotic area and tumor total area in experimental group was greater than that in control group (P<0.05).Conclusion The Survivin anti‐peptide have inhibitory effect on the development of tumors transplanted by human glioma U251 cells in nude rats.