CAJ | 학술논문

目的：探讨血清血小板衍生生长因子BB（ PDGF-BB）、可溶性血管内皮细胞生长因子受体-1（sFlt-1）、尿紧张素Ⅱ（U-Ⅱ）联合检测在子痫前期患者中的临床应用价值。方法采用病例对照研究，选取2012年10月至2014年4月期间入住聊城市第三人民医院产科96例子痫前期轻度患者、81例子痫前期重度患者及68名年龄和孕周匹配的正常孕妇。应用ELISA法检测血清中PDGF-BB、sFlt-1、U-Ⅱ水平。运用ROC曲线的AUC与二元回归logistic模型评价3项指标单独及联合检测对子痫前期患者的诊断价值。结果各组血清PDGF-BB、sFlt-1、U-Ⅱ浓度水平分别为：轻度组（80.45±21.87） ng／L、（23.03±6.67）μg／L、（4.54±1.02） ng／L；重度组（124.91±47.54） ng／L、（35.65±12.45）μg／L、（6.29±2.31）ng／L，对照组（60.89±19.38）ng／L、（17.19±7.86）μg／L、（3.81±1.01）ng／L。子痫前期轻度组、重度组3项指标均高于对照组，差异有统计学意义（ F值分别为79.43、79.28、50.72，P均＜0.01），子痫前期重度组3项指标均高于轻度组，差异均有统计学意义（P均＜0.01）。血清PDGF-BB、sFlt-1、U-Ⅱ单独及联合诊断子痫前期的AUC分别为0.821、0.786、0.772、0.933。3项指标联合检测敏感度95.7％，特异度86.6％，与3项指标单独检测差异有统计学意义（ P均＜0.05）。结论血清PDGF-BB、sFlt-1、U-Ⅱ的联合检测对子痫前期患者早期评估病情变化及诊治具有重要价值。（中华检验医学杂志，2015，38：528-531）
목적：탐토혈청혈소판연생생장인자BB（ PDGF-BB）、가용성혈관내피세포생장인자수체-1（sFlt-1）、뇨긴장소Ⅱ（U-Ⅱ）연합검측재자간전기환자중적림상응용개치。방법채용병례대조연구，선취2012년10월지2014년4월기간입주료성시제삼인민의원산과96례자간전기경도환자、81례자간전기중도환자급68명년령화잉주필배적정상잉부。응용ELISA법검측혈청중PDGF-BB、sFlt-1、U-Ⅱ수평。운용ROC곡선적AUC여이원회귀logistic모형평개3항지표단독급연합검측대자간전기환자적진단개치。결과각조혈청PDGF-BB、sFlt-1、U-Ⅱ농도수평분별위：경도조（80.45±21.87） ng／L、（23.03±6.67）μg／L、（4.54±1.02） ng／L；중도조（124.91±47.54） ng／L、（35.65±12.45）μg／L、（6.29±2.31）ng／L，대조조（60.89±19.38）ng／L、（17.19±7.86）μg／L、（3.81±1.01）ng／L。자간전기경도조、중도조3항지표균고우대조조，차이유통계학의의（ F치분별위79.43、79.28、50.72，P균＜0.01），자간전기중도조3항지표균고우경도조，차이균유통계학의의（P균＜0.01）。혈청PDGF-BB、sFlt-1、U-Ⅱ단독급연합진단자간전기적AUC분별위0.821、0.786、0.772、0.933。3항지표연합검측민감도95.7％，특이도86.6％，여3항지표단독검측차이유통계학의의（ P균＜0.05）。결론혈청PDGF-BB、sFlt-1、U-Ⅱ적연합검측대자간전기환자조기평고병정변화급진치구유중요개치。（중화검험의학잡지，2015，38：528-531）
Objective To explore the clinical diagnostic value of joint detection of serum platelet-derived growth factor BB (PDGF-BB), soluble vascular endothelial growth factor receptor -1 (sFlt-1) and urotensinⅡ( U-Ⅱ) in preeclampsia disease.Methods The cases of obstetric patients suffering from preeclampsia in the Third People′s Hospital of Liaocheng , Shandong Province between October 2012 and April 2014 were enrolled , including 96 cases of mild preeclampsia and 81 cases of severe preeclampsia.Totally 68 cases of normal pregnant women with similar age and gestational age were selected as control group.A case-control study was applied for the following investigations.The concentrations of serum PDGF-BB, sFlt-1 and U-Ⅱwere measured using ELISA.The diagnostic value of PDGF-BB, sFlt-1 and U-Ⅱalone or in combination for preeclampsia was analyzed and evaluated with receiver operating characteristic curve ( ROC) and Logistic regression analysis.Results Serum concentrations of PDGF-BB, sFlt-1 and U-Ⅱin mild preeclampsia group were (80.45 ±21.87)ng/L,(23.03 ±6.67)μg/L and(4.54 ± 1.02)ng/L, and those in severe preeclampsia group were (124.91 ±47.54)ng/L,(35.65 ±12.45)μg/L and(6.29 ±2.31) ng/L, while those in control group were (60.89 ±19.38) ng/L,(17.19 ±7.867)μg/L and ( 3.81 ±1.01 ) ng/L, respectively.The three parameters in mild preeclampsia group and severe preeclampsia group were significantly higher than those in control group ( P<0.01; F value was 79.43, 79.28 and 50.72 respectively ).In the same situation , these three indicators in severe preeclampsia group were significantly higher than those in mild group (P<0.01).Moreover, the AUC of serum PDGF-BB, sFlt-1 and U-Ⅱalone or in combination were 0.821, 0.786, 0.772 and 0.933, respectively.The differences between joint detection and three individual detections were statistically significant ( P<0.05 ) , as combined detection having a sensitivity of 95.7% and a specificity of 86.6%.Conclusion The combined detection of Serum PDGF-BB, sFlt-1 and U-Ⅱ had an important value in early assessment and treatment of preeclampsia.