CAJ | 학술논문

目的：分析慢性牙周炎患者龈沟液护骨素（ osteoprotegerin，OPG）和核因子κB受体活化因子配体（ receptor ac-tivator of nuclear factor-KB ligand，RANKL）表达与其龈沟出血指数的关系。方法以吸潮纸尖法，取37例慢性牙周炎患者（观察组）和同期入院体检的31例健康志愿者（对照组）的龈沟液。采用免疫荧光试验分析OPG和RANKL的表达强度；并以简单线性回归法测试OPG／RANKL表达与龈沟出血指数的相关性。结果与对照组比较，观察组患者OPG和OPG／RANKL表达强度明显偏低，RANKL表达强度明显偏高，且各指标的组间差异均有统计学意义（ P＜0．001）。经线性回归分析，观察组患者的 OPG／RANKL 的表达情况与其出血指数呈显著负相关（ y ＝－3．7755x＋3．6212，R2＝0．6651，P＜0．05）。结论 OPG／RANKL／RANK系统参与了慢性牙周炎的发生和发展，可作为衡量其炎症性出血程度的客观指标。
목적：분석만성아주염환자간구액호골소（ osteoprotegerin，OPG）화핵인자κB수체활화인자배체（ receptor ac-tivator of nuclear factor-KB ligand，RANKL）표체여기간구출혈지수적관계。방법이흡조지첨법，취37례만성아주염환자（관찰조）화동기입원체검적31례건강지원자（대조조）적간구액。채용면역형광시험분석OPG화RANKL적표체강도；병이간단선성회귀법측시OPG／RANKL표체여간구출혈지수적상관성。결과여대조조비교，관찰조환자OPG화OPG／RANKL표체강도명현편저，RANKL표체강도명현편고，차각지표적조간차이균유통계학의의（ P＜0．001）。경선성회귀분석，관찰조환자적 OPG／RANKL 적표체정황여기출혈지수정현저부상관（ y ＝－3．7755x＋3．6212，R2＝0．6651，P＜0．05）。결론 OPG／RANKL／RANK계통삼여료만성아주염적발생화발전，가작위형량기염증성출혈정도적객관지표。
Objective To examine the relationship between the level of osteoprotegerin ( OPG) and receptor activator of nuclear factor-KB ligand ( RANKL) expression and sulcus bleeding index.Method The gingival crevicular fluid of 37 patients with chronic periodontitis ( chosen as the experimental group ) and 31 healthy volunteers were abstracted with moisture absorption paper point method.Their OPG and RANKL expression level were detected with immunofluorescence test and the correlation of OPG/RANKL expression and sulcus bleeding index were analyzed with simple linear regression. Findings Compared with the control group, the OPG and OPG/RANKL expression level were lower significantly and the RANKL expression level were higher significantly (P<0.001).Negative correlation was found between the OPG/RANKL expression level and sulcus bleeding index (y=-3.7755x +3.6212,R2 =0.6651,P<0.05).Conclusion OPG/RANKL/RANK system is involved in the occurrence and development of chronic periodontitis.It can be used as an objective index to measure the degree of inflammatory bleeding.