CAJ | 학술논문

目的：建立慢性-非缓解的实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis，EAE）模型，以便用于多发性硬化症的研究和治疗。方法采用背部多点 MOG35-55免疫建立 EAE 模型，模型期间按 Benson 评分标准对 EAE 的病程进行临床评估，取发病高峰期脊髓和脑组织进行 H&E 染色，观察炎症细胞浸润情况。结果经该方法建立 C57BL/6J 小鼠 EAE 模型，具有稳定、发病率高的特点。对 EAE 小鼠的脊髓、脑组织进行的病理学检测结果表明，与对照组小鼠相比，EAE 小鼠的脊髓、脑组织中炎性细胞浸润增多。结论本研究成功构建了 MOG35-55免疫的 C57BL/6J EAE 小鼠模型。
목적：건립만성-비완해적실험성자신면역성뇌척수염（experimental autoimmune encephalomyelitis，EAE）모형，이편용우다발성경화증적연구화치료。방법채용배부다점 MOG35-55면역건립 EAE 모형，모형기간안 Benson 평분표준대 EAE 적병정진행림상평고，취발병고봉기척수화뇌조직진행 H&E 염색，관찰염증세포침윤정황。결과경해방법건립 C57BL/6J 소서 EAE 모형，구유은정、발병솔고적특점。대 EAE 소서적척수、뇌조직진행적병이학검측결과표명，여대조조소서상비，EAE 소서적척수、뇌조직중염성세포침윤증다。결론본연구성공구건료 MOG35-55면역적 C57BL/6J EAE 소서모형。
Objective To obtain chronic progressive EAE model used to study the pathogenesis and identify the novel drug target of multiple sclerosis. Methods The C57BL/ 6J mice were immunized with immunogen MOG35 - 55 by multi - point subcutane-ous injection in the hind flank. The clinical score assessment was performed daily according to Benson’s scoring criteria. And the histological examination of H&E - stained paraffin - embedded sections of spinal cord and brain harvested at peak of EAE was performed. Results A stable EAE model was successfully estab-lished with high incidence. Compared with the normal mice,inflammatory cell infiltrates were increased in the spinal cord and brain tissue of EAE mice. Conclusion We successfully established the MOG35 - 55 - induced EAE model in C57BL/ 6J mice.