中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2015年
8期
716-721
,共6页
胡豫杰%赫英英%王晔茹%崔生辉%陈秋霞%刘桂华%陈倩%周刚%杨保伟%黄金林%于红霞%李凤琴
鬍豫傑%赫英英%王曄茹%崔生輝%陳鞦霞%劉桂華%陳倩%週剛%楊保偉%黃金林%于紅霞%李鳳琴
호예걸%혁영영%왕엽여%최생휘%진추하%류계화%진천%주강%양보위%황금림%우홍하%리봉금
沙门菌属%环丙沙星%头孢噻肟%电泳,凝胶,脉冲场%耐药
沙門菌屬%環丙沙星%頭孢噻肟%電泳,凝膠,脈遲場%耐藥
사문균속%배병사성%두포새우%전영,응효,맥충장%내약
Salmonella%Ciprofloxacin%Cefotaxime%Electrophoresis,gel,pulsed-field%Drug resistance
目的：对中国6个省份零售整鸡中对环丙沙星与头孢噻肟双耐药的沙门菌流行状况及分子分型进行研究。方法对我国6个省份市售整鸡样品中分离的2629株沙门菌进行耐药性实验，筛选出对环丙沙星和头孢噻肟双重耐药的菌株，进行血清分型、超广谱β-内酰胺酶（ESBLs）表型确证和PFGE遗传特性研究。结果对环丙沙星与头孢噻肟双耐药的沙门菌共计227株（8.52%，227/2629），北京、吉林、广东、江苏、陕西、内蒙的检出率分别为11.67％（99/874）、8.20％（60/726）、1.39％（7/502）、15.61％（42/260）、8.56％（16/186）、0（0/81）；224株为印第安纳沙门菌，其中213株（95.10%）为ESBLs阳性。所有双耐印第安纳沙门菌均耐5种以上的抗生素，17.86%的菌株（40/224）对除碳青霉烯类外的10种抗生素均耐药；50.89%的菌株（114/224）对9种抗生素耐药，25.45%的菌株（57/224）对8种抗生素耐药。224株双耐印第安纳沙门菌划分为32个基因簇和150种PFGE带型，双耐印第安纳沙门菌带型既具有地域差异，相同或不同的省份及采样时间均发现相同带型菌株。结论中国零售整鸡中环丙沙星与头孢噻肟双耐药沙门菌污染严重，是双耐药沙门菌的重要储存库，分子分型结果提示沙门菌存在交叉污染或共同污染来源。
目的：對中國6箇省份零售整鷄中對環丙沙星與頭孢噻肟雙耐藥的沙門菌流行狀況及分子分型進行研究。方法對我國6箇省份市售整鷄樣品中分離的2629株沙門菌進行耐藥性實驗，篩選齣對環丙沙星和頭孢噻肟雙重耐藥的菌株，進行血清分型、超廣譜β-內酰胺酶（ESBLs）錶型確證和PFGE遺傳特性研究。結果對環丙沙星與頭孢噻肟雙耐藥的沙門菌共計227株（8.52%，227/2629），北京、吉林、廣東、江囌、陝西、內矇的檢齣率分彆為11.67％（99/874）、8.20％（60/726）、1.39％（7/502）、15.61％（42/260）、8.56％（16/186）、0（0/81）；224株為印第安納沙門菌，其中213株（95.10%）為ESBLs暘性。所有雙耐印第安納沙門菌均耐5種以上的抗生素，17.86%的菌株（40/224）對除碳青黴烯類外的10種抗生素均耐藥；50.89%的菌株（114/224）對9種抗生素耐藥，25.45%的菌株（57/224）對8種抗生素耐藥。224株雙耐印第安納沙門菌劃分為32箇基因簇和150種PFGE帶型，雙耐印第安納沙門菌帶型既具有地域差異，相同或不同的省份及採樣時間均髮現相同帶型菌株。結論中國零售整鷄中環丙沙星與頭孢噻肟雙耐藥沙門菌汙染嚴重，是雙耐藥沙門菌的重要儲存庫，分子分型結果提示沙門菌存在交扠汙染或共同汙染來源。
목적：대중국6개성빈령수정계중대배병사성여두포새우쌍내약적사문균류행상황급분자분형진행연구。방법대아국6개성빈시수정계양품중분리적2629주사문균진행내약성실험，사선출대배병사성화두포새우쌍중내약적균주，진행혈청분형、초엄보β-내선알매（ESBLs）표형학증화PFGE유전특성연구。결과대배병사성여두포새우쌍내약적사문균공계227주（8.52%，227/2629），북경、길림、엄동、강소、협서、내몽적검출솔분별위11.67％（99/874）、8.20％（60/726）、1.39％（7/502）、15.61％（42/260）、8.56％（16/186）、0（0/81）；224주위인제안납사문균，기중213주（95.10%）위ESBLs양성。소유쌍내인제안납사문균균내5충이상적항생소，17.86%적균주（40/224）대제탄청매희류외적10충항생소균내약；50.89%적균주（114/224）대9충항생소내약，25.45%적균주（57/224）대8충항생소내약。224주쌍내인제안납사문균화분위32개기인족화150충PFGE대형，쌍내인제안납사문균대형기구유지역차이，상동혹불동적성빈급채양시간균발현상동대형균주。결론중국령수정계중배병사성여두포새우쌍내약사문균오염엄중，시쌍내약사문균적중요저존고，분자분형결과제시사문균존재교차오염혹공동오염래원。
Objective To elucidate the epidemic condition and molecular subtyping of ciprofloxacin and cefotaxime co-resistant Salmonella Indiana(S. Indiana)isolated from retail chicken carcasses in six provinces of China. Methods A total of 2 647 Salmonella strains isolated from retail chicken carcasses collected from six provinces of China were subjected to antimicrobial susceptibility testing. All Salmonella isolates co-resistant to ciprofloxacin and cefotaxime were further characterized by serotyping, extended-spectrum beta-lactamases (ESBLs) producing strains screening and pulsed field gel electrophoresis (PFGE) typing. Results Among 2 629 Salmonella isolates tested, 227 (8.52%) isolates were co-resistant to ciprofloxacin and ceftazidime/cefotaxime (Beijing:11.67%(99/874),Jilin:8.20%(60/726), Guangdong: 1.39%(7/502),Jiangsu: 15.61%(42/260),Shaanxi: 8.56%(16/186),Inner Mongolia: 0(0/81)), and 224 of them were identified as S. Indiana. 213(95.10%)isolates of S. Indiana were ESBLs producing strains. All ciprofloxacin and cefotaxime co-resistant S. Indiana isolates developed a multi-drug resistant profile and 17.86%(40/224)of them were resistant to all antibiotics tested except carbapenems, and 50.89%(114/224)of them resistant to 9 antibiotics, additionally, 25.45%(57/224)of them showed multi-drug resistance to 8 antibiotics. All ciprofloxacin and cefotaxime co-resistant S. Indiana isolates were divided into 32 PFGE clusters and 150 PFGE patterns. Strains of S. Indiana from same or different sampling site and time seemed to either share the same PFGE patterns or be differential to each other in different regions. Conclusion The results indicated that chicken carcasses collected from parts of China were heavily contaminated by ciprofloxacin and cefotaxime co-resistant S. Indiana and could serve as an important reservoir of ciprofloxacin and cefotaxime co-resistant Salmonella. Molecular subtyping results indicated that cross contamination or common pollution source might be in these strains.
