CAJ | 학술논문

利用显微电子成像技术对长鳍吻(Rhinogobio ventralis)胚胎发育和仔鱼形态进行了观察与研究，详细描述了各发育期的形态特征和发育时间。结果显示，长鳍吻卵子的卵径为1.7–1.9 mm、卵膜径为6.6–7.0 mm、卵粒质量为0.0059–0.0077 g，精子的寿命为96.56–119.12 s、激烈运动时间为25.43–31.43 s。长鳍吻的胚胎发育可分为受精卵、胚盘期、卵裂期、囊胚期、原肠期、神经胚期、器官形成期和出膜期8个阶段，在水温为17.6–18.3℃流水孵化条件下，其受精卵历时56 h 孵化出膜，在水温为18.5–22.0℃的条件下，长鳍吻仔鱼的卵黄囊在5日龄时基本消失，6日龄时完全消失，进入后期仔鱼阶段，30日龄的长鳍吻仔鱼鳍条基本形成，鳞片开始形成，结束仔鱼期，进入稚鱼期。
이용현미전자성상기술대장기문(Rhinogobio ventralis)배태발육화자어형태진행료관찰여연구，상세묘술료각발육기적형태특정화발육시간。결과현시，장기문란자적란경위1.7–1.9 mm、란막경위6.6–7.0 mm、란립질량위0.0059–0.0077 g，정자적수명위96.56–119.12 s、격렬운동시간위25.43–31.43 s。장기문적배태발육가분위수정란、배반기、란렬기、낭배기、원장기、신경배기、기관형성기화출막기8개계단，재수온위17.6–18.3℃류수부화조건하，기수정란력시56 h 부화출막，재수온위18.5–22.0℃적조건하，장기문자어적란황낭재5일령시기본소실，6일령시완전소실，진입후기자어계단，30일령적장기문자어기조기본형성，린편개시형성，결속자어기，진입치어기。
The current study investigated the embryonic and early larval development of Rhinogobio ventralis using the micro electronic imaging technology. The running-water hatching way was used to incubate the fertilized egg. The hatching water temperature was 17.6-18.3℃ and DO was 7-9 mg/L. The results showed that the range of R.ventralis egg diameter,egg membrane diameter and egg weight was 1.7-1.9 mm, 6.6-7.0 mm and 0.0059-0.0077 g, respectively, and that the life and swirling movement time of sperm were 96.56-119.12 s and 25.43-31.43 s, respectively. The time period for embryonic development was 56 h at 17.6-18.3℃. Eight embryo development stages of R.ventralis were described, which included fertilized egg, blastoderm, cleavage, blastula, gastrula, neurula, organogenesis, and hatching period. The yolk-sac of larvae disappeared after being reared for 5 days in the water at 18.5-22℃. The yolk-sac of 6-days age larvae disappeared completely, and the late larval stage began. The fin of 30-days age larva was basically formed and scales began to form. The growing larvae further developed into juvenile stage after 30 days. Our study provides theoretical basis and scientific reference for the mass reproduction of R.ventralis.