渔业科学进展
漁業科學進展
어업과학진전
MARINE FISHERIES RESEARCH
2015年
4期
83-88
,共6页
杨宁%姜芳燕%黄海%焦健
楊寧%薑芳燕%黃海%焦健
양저%강방연%황해%초건
罗非鱼%简达气单胞菌%致病性%分离鉴定%药敏试验
囉非魚%簡達氣單胞菌%緻病性%分離鑒定%藥敏試驗
라비어%간체기단포균%치병성%분리감정%약민시험
Tilapia nilotica%Aeromonas jandaei%Pathogenicity%Isolation and identification%Sensitivity test
从患病罗非鱼(Tilapia nilotica)体内分离得到细菌 NL05,通过回归感染试验确定 NL05为致病菌,并测出 NL05对罗非鱼的半致死量(LD50)为1×103 CFU/g。结合细菌形态学特征、生理生化指标和16S rRNA 基因同源分析,鉴定 NL05为简达气单胞菌(Aeromonas jandaei)。形态学观察发现, NL05为革兰氏阴性、短杆状;生理生化试验中麦芽糖、甘露醇、葡萄糖、水杨素、硫化氢等13种指标为阳性,蔗糖、阿拉伯糖、木糖、肌醇、卫矛醇等10种指标为阴性。药敏试验显示,NL05对奥复星、丙氟哌酸、丁胺卡那霉素、多粘菌素 B、氟哌酸、利福平、洁霉素等13种抗生素敏感,对阿奇霉素、菌必治、卡那霉素、链霉素、美满霉素5种抗生素中介,对氨苄青霉素、阿莫西林、恩诺沙星、复方新诺明、甲氧苄啶等13种抗生素耐药。
從患病囉非魚(Tilapia nilotica)體內分離得到細菌 NL05,通過迴歸感染試驗確定 NL05為緻病菌,併測齣 NL05對囉非魚的半緻死量(LD50)為1×103 CFU/g。結閤細菌形態學特徵、生理生化指標和16S rRNA 基因同源分析,鑒定 NL05為簡達氣單胞菌(Aeromonas jandaei)。形態學觀察髮現, NL05為革蘭氏陰性、短桿狀;生理生化試驗中麥芽糖、甘露醇、葡萄糖、水楊素、硫化氫等13種指標為暘性,蔗糖、阿拉伯糖、木糖、肌醇、衛矛醇等10種指標為陰性。藥敏試驗顯示,NL05對奧複星、丙氟哌痠、丁胺卡那黴素、多粘菌素 B、氟哌痠、利福平、潔黴素等13種抗生素敏感,對阿奇黴素、菌必治、卡那黴素、鏈黴素、美滿黴素5種抗生素中介,對氨芐青黴素、阿莫西林、恩諾沙星、複方新諾明、甲氧芐啶等13種抗生素耐藥。
종환병라비어(Tilapia nilotica)체내분리득도세균 NL05,통과회귀감염시험학정 NL05위치병균,병측출 NL05대라비어적반치사량(LD50)위1×103 CFU/g。결합세균형태학특정、생리생화지표화16S rRNA 기인동원분석,감정 NL05위간체기단포균(Aeromonas jandaei)。형태학관찰발현, NL05위혁란씨음성、단간상;생리생화시험중맥아당、감로순、포도당、수양소、류화경등13충지표위양성,자당、아랍백당、목당、기순、위모순등10충지표위음성。약민시험현시,NL05대오복성、병불고산、정알잡나매소、다점균소 B、불고산、리복평、길매소등13충항생소민감,대아기매소、균필치、잡나매소、련매소、미만매소5충항생소중개,대안변청매소、아막서림、은낙사성、복방신낙명、갑양변정등13충항생소내약。
Tilapia is a very important freshwater aquacultural species in China; however, bacterial disease caused huge economic losses in recent years with the continous expansion of tilapia aquaculture. The aim of this research was to isolate, identify, and test drug sensitivity of bacteria pathogen found in diseased Tilapia nilotica. Six bacteria were isolated from diseased T. nilotica as suspected pathogenic bacteria. The bacteria (NL05) isolated from intestine of diseased T. nilotica was determined as pathogenic bacteria by infection experiment. The LD50 was 1×103 CFU/g. Bacteria NL05 was identified as Aeromonas jandaei. Morphology observation indicated that NL05 was gram-negative, rod-shaped. Thirteen physiological and biochemical indexes were positive including maltose, mannitol, glucose, salicin, and hydrogen sulfide. Ten indexes were negative including saccharum, arabinose, xylose, inositol, and dulcitol. There was 1442 bp in the sequence of 16S rRNA, which was submitted into GenBank (Accession number: KC916744). The phylogenetic tree based on 16S rRNA showed that NL05 was A. jandaei. Drug sensitivity test indicated that NL05 was sensitive to 13 antibiotics including ofloxacin, ciprofloxacin, amikacin, polymyxin B, norfloxacin, rifampicin, and lincomycin, and intermediate sensitive to 5 antibiotics including azithromycin, ceftriaxone, kanamycin, streptomycin and minocycline, and resistant to 13 antibiotics including ampicillin, amoxicillin, enrofloxacin, sulfamethoxazole, and trimethoprim. The results will provide references for the prevention and treatment of diseases caused by T. nilotica.