中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
32期
2599-2602
,共4页
郭玮%宋斌斌%沈隽霏%吴炯%张春燕%王蓓丽%潘柏申
郭瑋%宋斌斌%瀋雋霏%吳炯%張春燕%王蓓麗%潘柏申
곽위%송빈빈%침준비%오형%장춘연%왕배려%반백신
生物参考区间%丙氨酸转氨酶%间接法%参考变化值
生物參攷區間%丙氨痠轉氨酶%間接法%參攷變化值
생물삼고구간%병안산전안매%간접법%삼고변화치
Biological reference interval%Alanine transaminase%Indirect method%Reference change value
目的 利用实验室信息系统(LIS)中储存的丙氨酸转氨酶(ALT)检测结果,建立一种以数学统计为基础的间接法生物参考区间获取模式.方法 调取2014年门诊及健康体检人群的ALT检测结果,通过Box-Cox的数据正态性转换、分别使用四分位间距法和肖维纳准则进行离群值的剔除,同时使用非参数排序法和Hoffmann计算法得到参考区间,并引用参考变化值(RCV)为判断标准与直接法结果进行比较.结果 正态性转换后的ALT数据,使用四分位间距法联合Hoffmann计算法得到的参考区间分别为:门诊男性12~41 U/L;体检男性12~48 U/L;门诊女性9~32 U/L;体检女性8~ 35 U/L,与直接法参考区间进行比较,差异均小于RCV(54%).结论 Box-Cox转换结合四分位间距法和Hoffmann计算法是一种简单且可靠的间接法参考区间获取模式,适用于临床实验室的推广和应用.
目的 利用實驗室信息繫統(LIS)中儲存的丙氨痠轉氨酶(ALT)檢測結果,建立一種以數學統計為基礎的間接法生物參攷區間穫取模式.方法 調取2014年門診及健康體檢人群的ALT檢測結果,通過Box-Cox的數據正態性轉換、分彆使用四分位間距法和肖維納準則進行離群值的剔除,同時使用非參數排序法和Hoffmann計算法得到參攷區間,併引用參攷變化值(RCV)為判斷標準與直接法結果進行比較.結果 正態性轉換後的ALT數據,使用四分位間距法聯閤Hoffmann計算法得到的參攷區間分彆為:門診男性12~41 U/L;體檢男性12~48 U/L;門診女性9~32 U/L;體檢女性8~ 35 U/L,與直接法參攷區間進行比較,差異均小于RCV(54%).結論 Box-Cox轉換結閤四分位間距法和Hoffmann計算法是一種簡單且可靠的間接法參攷區間穫取模式,適用于臨床實驗室的推廣和應用.
목적 이용실험실신식계통(LIS)중저존적병안산전안매(ALT)검측결과,건립일충이수학통계위기출적간접법생물삼고구간획취모식.방법 조취2014년문진급건강체검인군적ALT검측결과,통과Box-Cox적수거정태성전환、분별사용사분위간거법화초유납준칙진행리군치적척제,동시사용비삼수배서법화Hoffmann계산법득도삼고구간,병인용삼고변화치(RCV)위판단표준여직접법결과진행비교.결과 정태성전환후적ALT수거,사용사분위간거법연합Hoffmann계산법득도적삼고구간분별위:문진남성12~41 U/L;체검남성12~48 U/L;문진녀성9~32 U/L;체검녀성8~ 35 U/L,여직접법삼고구간진행비교,차이균소우RCV(54%).결론 Box-Cox전환결합사분위간거법화Hoffmann계산법시일충간단차가고적간접법삼고구간획취모식,괄용우림상실험실적추엄화응용.
Objective To establish an indirect reference interval based on the test results of alanine aminotransferase stored in a laboratory information system.Methods All alanine aminotransferase results were included for outpatients and physical examinations that were stored in the laboratory information system of Zhongshan Hospital during 2014.The original data were transformed using a Box-Cox transformation to obtain an approximate normal distribution.Outliers were identified and omitted using the Chauvenet and Tukey methods.The indirect reference intervals were obtained by simultaneously applying nonparametric and Hoffmann methods.The reference change value was selected to determine the statistical significance of the observed differences between the calculated and published reference intervals.Results The indirect reference intervals for alanine aminotransferase of all groups were 12 to 41 U/L (male,outpatient),12 to 48 U/L (male,physical examination),9 to 32 U/L (female,outpatient),and 8 to 35 U/L (female,physical examination),respectively.The absolute differences when compared with the direct results were all smaller than the reference change value of alanine aminotransferase.Conclusion The Box-Cox transformation combined with the Hoffmann and Tukey methods is a simple and reliable technique that should be promoted and used by clinical laboratories.