河南医学研究
河南醫學研究
하남의학연구
HENAN MEDICAL RESEARCH
2015年
8期
1-5
,共5页
李芳%黄瓅%苏晓丽%胡成平
李芳%黃瓅%囌曉麗%鬍成平
리방%황력%소효려%호성평
EGCG%乏氧%肺腺癌%顺铂
EGCG%乏氧%肺腺癌%順鉑
EGCG%핍양%폐선암%순박
EGCG%hypoxia%lung adenocarcinoma%cisplatin
目的:探讨 EGCG 联合顺铂对乏氧人肺腺癌 A549细胞凋亡的影响。方法采用氯化钴(CoCl2)建立人肺腺癌 A549细胞体外化学乏氧模型,分为乏氧组、EGCG +乏氧组及常氧组。MTT 法、光镜下 Hoechst 染色法、AnnexinV -FITC/ PI 双染法测定 EGCG 单独或联合顺铂时对乏氧 A549细胞生长、细胞核形态及凋亡率的影响。结果200μmol/ L CoCl2乏氧条件下,加入0、25、50、100、150、200 mg/ L EGCG 作用48 h 后,以50 mg/ L EGCG 为拐点,A549细胞生长抑制率呈 EGCG 浓度依赖性增加。与0 mg/ L EGCG 相比,50 mg/ L EGCG 作用时,部分乏氧 A549细胞核形态不规则,染色质固缩、致密浓染,凋亡率由(0.41%±0.14%)增加至(3.15%±0.28%)(P <0.05);顺铂 IC50由(107.01±10.19)μmol/ L降低至(41.28±3.85)μmol/ L(P <0.05);与10μmol/ L 顺铂联合时,A549细胞凋亡率由(8.12%±0.67%)增加至(13.28%±3.11%)(P <0.05)。结论 EGCG 联合顺铂对乏氧 A549细胞的凋亡起促进作用。
目的:探討 EGCG 聯閤順鉑對乏氧人肺腺癌 A549細胞凋亡的影響。方法採用氯化鈷(CoCl2)建立人肺腺癌 A549細胞體外化學乏氧模型,分為乏氧組、EGCG +乏氧組及常氧組。MTT 法、光鏡下 Hoechst 染色法、AnnexinV -FITC/ PI 雙染法測定 EGCG 單獨或聯閤順鉑時對乏氧 A549細胞生長、細胞覈形態及凋亡率的影響。結果200μmol/ L CoCl2乏氧條件下,加入0、25、50、100、150、200 mg/ L EGCG 作用48 h 後,以50 mg/ L EGCG 為枴點,A549細胞生長抑製率呈 EGCG 濃度依賴性增加。與0 mg/ L EGCG 相比,50 mg/ L EGCG 作用時,部分乏氧 A549細胞覈形態不規則,染色質固縮、緻密濃染,凋亡率由(0.41%±0.14%)增加至(3.15%±0.28%)(P <0.05);順鉑 IC50由(107.01±10.19)μmol/ L降低至(41.28±3.85)μmol/ L(P <0.05);與10μmol/ L 順鉑聯閤時,A549細胞凋亡率由(8.12%±0.67%)增加至(13.28%±3.11%)(P <0.05)。結論 EGCG 聯閤順鉑對乏氧 A549細胞的凋亡起促進作用。
목적:탐토 EGCG 연합순박대핍양인폐선암 A549세포조망적영향。방법채용록화고(CoCl2)건립인폐선암 A549세포체외화학핍양모형,분위핍양조、EGCG +핍양조급상양조。MTT 법、광경하 Hoechst 염색법、AnnexinV -FITC/ PI 쌍염법측정 EGCG 단독혹연합순박시대핍양 A549세포생장、세포핵형태급조망솔적영향。결과200μmol/ L CoCl2핍양조건하,가입0、25、50、100、150、200 mg/ L EGCG 작용48 h 후,이50 mg/ L EGCG 위괴점,A549세포생장억제솔정 EGCG 농도의뢰성증가。여0 mg/ L EGCG 상비,50 mg/ L EGCG 작용시,부분핍양 A549세포핵형태불규칙,염색질고축、치밀농염,조망솔유(0.41%±0.14%)증가지(3.15%±0.28%)(P <0.05);순박 IC50유(107.01±10.19)μmol/ L강저지(41.28±3.85)μmol/ L(P <0.05);여10μmol/ L 순박연합시,A549세포조망솔유(8.12%±0.67%)증가지(13.28%±3.11%)(P <0.05)。결론 EGCG 연합순박대핍양 A549세포적조망기촉진작용。
Objective To investigate the effect of EGCG in combination with cisplatin on apoptosis of human lung adenocarci-noma A549 cells under hypoxia conditions. Methods Cobalt chloride(CoCl2 )was used to establish chemical hypoxia model of A549 cell in vitro. Proliferation,nuclear morphology and apoptosis rate of A549 cell under EGCG alone or in combination with cisplatin were measured by MTT,Hoechst staining and AnnexinV - FITC / PI method,respectively. Results after cultured in 0,25,50,100,150,200 mg/ L EGCG for 48 hours,growth inhibition rates of hypoxic A549 cell increased in a EGCG dose de-pendent manner,with 50 mg/ L as the inflection point. Compared with the 0 mg/ L EGCG,50 mg / L EGCG made the nuclear of some A549 irregular with chromatin pyknosis and condensed,accompanied apoptotic rate increased to(3. 15% ± 0. 28% ) from(0. 41% ± 0. 14% )(P < 0. 05),IC50 of cisplatin decreased from(107. 01 ± 10. 19)μmol/ L to(41. 28 ± 3. 85)μmol/ L (P < 0. 05),when combined with 10 μmol/ L cisplatin,the apoptotic rate increased to(13. 28% ± 3. 11% )from(8. 12% ± 0. 67% )(P < 0. 05). Conclusion EGCG in combination with cisplatin could promote the apoptosis rate of A549 cell under hy-poxic condition.