CAJ | 학술논문

目的：建立测定肾茶提取物中橙黄酮、半齿泽兰素、半齿泽兰素-5-甲醚含量的方法。方法采用高效液相色谱(HPLC)法,Symmetry C18(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-0.05％磷酸缓冲液,梯度洗脱；流速：1 mL?min－1,柱温：30℃,检测波长：365 nm,进样量为10μL。结果橙黄酮在0.50~5.00μg、半齿泽兰素在0.50~5.00μg、半齿泽兰素-5-甲醚在0.05~0.50μg 范围内具有良好的线性关系；平均加样回收率分别为101.26％,100.28％和99.66％。 RSD 分别为1.73％,0.82％和1.67％。结论该方法简便、准确,可用于肾茶的质量评价。
목적：건립측정신다제취물중등황동、반치택란소、반치택란소-5-갑미함량적방법。방법채용고효액상색보(HPLC)법,Symmetry C18(4.6 mm×150 mm,5μm)색보주,류동상위을정-0.05％린산완충액,제도세탈；류속：1 mL?min－1,주온：30℃,검측파장：365 nm,진양량위10μL。결과등황동재0.50~5.00μg、반치택란소재0.50~5.00μg、반치택란소-5-갑미재0.05~0.50μg 범위내구유량호적선성관계；평균가양회수솔분별위101.26％,100.28％화99.66％。 RSD 분별위1.73％,0.82％화1.67％。결론해방법간편、준학,가용우신다적질량평개。
Objective To develop a method for content determination of sinensetin,eupatorin,and 3′-hydroxy-5,6,7, 4′-tetramethoxyflavone in Orthosiphon stamineus. Methods The determination was carried out on a Symmetry C18 column (4.6 mm×250 mm,5 μm) by HPLC.The mobile consisted of acetonitrile and water containing 0.05% H3 PO4 in gradient elution. The flow rate was 1 mL?min-1 ,the column temperature was 30 ℃ ,the detected wavelength was set at 365 nm, and the injection volume was 10 μL. Results The peak areas and the sample quantity of the three components presented good linear relationship in the range of 0. 50 - 5. 00 μg for sinensetin,0. 50 - 5. 00 μg for eupatorin, and 0. 05 - 0. 50 μg for 3′-hydroxy-5,6,7,4′-tetramethoxyflavone.The average recoveries were 101.26%,100.28% and 99.66%,respectively. RSD were 1.73%, 0.82% and 1.67%, respectively. Conclusion The method is proved to be simple,accurate and can be used for the quality evaluation of Orthosiphon stamineus.