CAJ | 학술논문

目的：分析中晚期非小细胞肺癌患者化疗前后外周血中 Treg 细胞和 Th17细胞比例变化及临床意义。方法收集51例中晚期非小细胞肺癌患者（观察组）化疗前后及47例健康对照组外周血标本，应用流式细胞术检测 Treg 细胞和 Th17细胞所占 CD4+ T 细胞的比例，观察 Treg 和 Th17细胞比例在化疗前后的变化及其相关性。结果化疗后缓解组外周血中 Th17及 Treg 细胞比例较化疗前降低（P <0.05），Treg 细胞仍高于对照组（P <0.05），Th17细胞比例稍高于对照组（P >0.05）。非缓解组2种细胞的比例较化疗前增高（P <0.05），并明显高于对照组（ P <0.05）。无论是化疗前，还是化疗后，非小细胞肺癌患者外周血中Th17与 Treg 细胞之间均存在正相关关系（r =0.589、0.657，P <0.05）。结论化疗能够降低非小细胞肺癌患者外周血中 Th17及 Treg 细胞比例，对肿瘤免疫功能具有积极意义。
목적：분석중만기비소세포폐암환자화료전후외주혈중 Treg 세포화 Th17세포비례변화급림상의의。방법수집51례중만기비소세포폐암환자（관찰조）화료전후급47례건강대조조외주혈표본，응용류식세포술검측 Treg 세포화 Th17세포소점 CD4+ T 세포적비례，관찰 Treg 화 Th17세포비례재화료전후적변화급기상관성。결과화료후완해조외주혈중 Th17급 Treg 세포비례교화료전강저（P <0.05），Treg 세포잉고우대조조（P <0.05），Th17세포비례초고우대조조（P >0.05）。비완해조2충세포적비례교화료전증고（P <0.05），병명현고우대조조（ P <0.05）。무론시화료전，환시화료후，비소세포폐암환자외주혈중Th17여 Treg 세포지간균존재정상관관계（r =0.589、0.657，P <0.05）。결론화료능구강저비소세포폐암환자외주혈중 Th17급 Treg 세포비례，대종류면역공능구유적겁의의。
Objective To explore the changes of Th17 and Treg cells in the peripheral blood of patients with ad-vanced non-small cell lung cancer before and after chemotherapy,and to observe the clinical significance. Methods Flow cytometry was used to detect the proportion of Th17 and Treg cells in the peripheral blood of 51 patients with advanced non-small cell lung cancer(the observation group)before and after chemotherapy and 47 healthy volun-teers(the control group),and the correlation was observed too. Results The proportion of Th17 and Treg cells of the remission group after chemotherapy were all significantly lower than before chemotherapy(P < 0. 05). Compared with the control group,the proportion of Th17 cells of the remission group did not change significantly(P > 0. 05), but the proportion of Treg cells of the remission group was higher significantly(P < 0. 05). The proportion of Th17 and Treg cells of the non-remission group after chemotherapy were all significantly higher than before chemotherapy (P < 0. 05),and were higher than those of the control group(P < 0. 05). The proportion of Th17 cells were posi-tively related to the proportion of Treg cells in the peripheral blood of patients with advanced non-small cell lung cancer before and after chemotherapy(r = 0. 589,0. 657;P < 0. 05). Conclusion Chemotherapy can reduce the proportion of Th17 cells and Treg cells in the peripheral blood of patients with advanced non-small cell lung cancer, and has the positive significance for tumor immune function.