CAJ | 학술논문

目的：构建并鉴定含人 CXCR4基因启动子的荧光素酶的报告载体。方法采用克隆人 CXCR4基因启动子的序列，构建报告载体 PgL4.17-CXCR4，以其转染 Jurkat 细胞，然后测定荧光素酶的活性。结果扩增得到的 CXCR4启动子序列与克隆人 PgL4.17-CXCR4的 CXCR4启动子序列和 GenBank 报道的一致。实验组荧光素酶的表达活性是874809.0200±39510.6246，与空白组的465.9000±26.2501和对照组的37981.9800±2384.3412总体比较差异有统计学意义（F =4678.919，P <0.001）。结论按照本实验方案，含人 CXCR4基因启动子的荧光素酶的 PgL4.17-CXCR4报告载体被成功构建。
목적：구건병감정함인 CXCR4기인계동자적형광소매적보고재체。방법채용극륭인 CXCR4기인계동자적서렬，구건보고재체 PgL4.17-CXCR4，이기전염 Jurkat 세포，연후측정형광소매적활성。결과확증득도적 CXCR4계동자서렬여극륭인 PgL4.17-CXCR4적 CXCR4계동자서렬화 GenBank 보도적일치。실험조형광소매적표체활성시874809.0200±39510.6246，여공백조적465.9000±26.2501화대조조적37981.9800±2384.3412총체비교차이유통계학의의（F =4678.919，P <0.001）。결론안조본실험방안，함인 CXCR4기인계동자적형광소매적 PgL4.17-CXCR4보고재체피성공구건。
Objective To construct and identify a luciferase reporter vector PgL4. 17-CXCR4 containing human CXCR4 promoter. Methods The sequence of CXCR4 promoter gene was cloned,and was inserted into the reporter vector PgL4. 17 to construct luciferase reporter vector PgL4. 17-CXCR4 ,the recombinant plasmids were transfected into Jurkat cells. The luciferase activity was evaluated. Results CXCR4 promoter gene fragment was amplified by PCR. The insertion sequence of CXCR4 promoter in the experimental group was 874 809. 020 0 ± 39 510. 624 6,was 465. 900 0 ± 26. 250 1 in the blank group,and was 37 981. 980 0 ± 2 384. 341 2 in the control group( F =4 678. 919,P < 0. 001). Conclusion Luciferase reporter vector PgL4. 17-CXCR4 containing human CXCR4 pro-moter can be constructed successfully.