CAJ | 학술논문

目的探索苯职业接触者细胞色素氧化酶P450 2E1 (CYP2E1)Rsa Ⅰ/Pst Ⅰ与Dra Ⅰ基因多态性.方法采用横断面调查的方法,选择71名苯接触人群为观察组,以71名无职业性苯接触的人员为对照组.采集两组研究对象血样,应用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法对其CYP2E1 Rsa Ⅰ/Pst Ⅰ与Dra Ⅰ基因多态性进行研究分型.结果观察组与对照组CYP2E1 Dra Ⅰ位点基因型和等位基因的分布差异无统计学意义(基因型x2=2.374,P＞0.05;等位基因x2=2.113,P＞0.05);观察组与对照组CYP2E1 Rsa Ⅰ/Pst Ⅰ位点的基因型与等位基因分布差异均有统计学意义(基因型x2=9.129,P＜0.01;等位基因x2=6.028,P＜0.05).结论 CYP2E1 Rsa Ⅰ/Pst Ⅰ位点突变可增强体内CYP2E1的表达,提示携带该基因的个体对慢性苯中毒的易感性升高.
목적 탐색분직업접촉자세포색소양화매P450 2E1 (CYP2E1)Rsa Ⅰ/Pst Ⅰ여Dra Ⅰ기인다태성.방법 채용횡단면조사적방법,선택71명분접촉인군위관찰조,이71명무직업성분접촉적인원위대조조.채집량조연구대상혈양,응용한제성편단장도다태성취합매련반응(PCR-RFLP)방법대기CYP2E1 Rsa Ⅰ/Pst Ⅰ여Dra Ⅰ기인다태성진행연구분형.결과 관찰조여대조조CYP2E1 Dra Ⅰ위점기인형화등위기인적분포차이무통계학의의(기인형x2=2.374,P＞0.05;등위기인x2=2.113,P＞0.05);관찰조여대조조CYP2E1 Rsa Ⅰ/Pst Ⅰ위점적기인형여등위기인분포차이균유통계학의의(기인형x2=9.129,P＜0.01;등위기인x2=6.028,P＜0.05).결론 CYP2E1 Rsa Ⅰ/Pst Ⅰ위점돌변가증강체내CYP2E1적표체,제시휴대해기인적개체대만성분중독적역감성승고.
Objective To investigate the cytochrome P450 2E1 (CYP2E1) RsaⅠ/PstⅠ and DraⅠ polymorphisms in workers exposed to benzene.Methods A cross-sectional survey was carried out.A total of 71 workers exposed to benzene were included in observation group and the same number of people without occupational benzene exposure were included in control group.Blood samples from the two groups were collected and genotyping for CYP2E1 RsaⅠ/PstⅠ and DraⅠ were conducted using the polymerase chain reactionrestriction fragment length polymorphism.Results There were no significant differences in CYP2E1 DraⅠ genotype and allele distributions between the observation group and the control group (x2=2.374,P＞0.05; x2=2.113,P＞0.05).Significant differences in CYP2E1 RsaⅠ/PstⅠ genotype and allele distributions between the two groups were observed (x2=9.129,P＜0.01; x2=6.028,P＜0.05).Conclusion Mutations at CYP2E 1 RsaⅠ/PstⅠ can enhance the expression of CYP2E1 and this suggests individuals with the mutated gene have increased susceptibility to chronic benzene poisoning.