CAJ | 학술논문

为分析添加不同促溶剂（NaCl和木瓜蛋白酶）所得酵母细胞自溶物的成分差别，采用气相色谱-质谱联用（GC-MS）技术，对酵母细胞自溶物乙酸乙酯萃取物中的化学成分进行分析测定，结合计算机检索技术对其成分进行结构鉴定，应用色谱峰面积归一化法计算各成分的相对百分含量。结果表明，添加NaCl所得自溶物的乙酸乙酯萃取物共分离出49种成分，鉴定出28种，占总量的92.14%，其中酯类3种，酸类5种，醇类4种，杂环类6种（吡咯、呋喃、吡咯并吡嗪、嘧啶、吡唑等），酚类2种，饱和芳香烃3种，饱和脂肪烃5种；添加木瓜蛋白酶所得自溶物的乙酸乙酯萃取物中共分离出49种成分，鉴定出27种，占总量的91.006%，其中酯类2种，酸类4种，醇类4种，杂环类7种（吡咯、呋喃、吡咯并吡嗪、嘧啶、噻唑、吡喃等），酚类5种，饱和芳香烃2种，不饱和芳香烃2种，腈类1种。由此可见，不同处理方式所得酵母细胞自溶物乙酸乙酯萃取物共有21种成分，其余成分需进一步研究。
위분석첨가불동촉용제（NaCl화목과단백매）소득효모세포자용물적성분차별，채용기상색보-질보련용（GC-MS）기술，대효모세포자용물을산을지췌취물중적화학성분진행분석측정，결합계산궤검색기술대기성분진행결구감정，응용색보봉면적귀일화법계산각성분적상대백분함량。결과표명，첨가NaCl소득자용물적을산을지췌취물공분리출49충성분，감정출28충，점총량적92.14%，기중지류3충，산류5충，순류4충，잡배류6충（필각、부남、필각병필진、밀정、필서등），분류2충，포화방향경3충，포화지방경5충；첨가목과단백매소득자용물적을산을지췌취물중공분리출49충성분，감정출27충，점총량적91.006%，기중지류2충，산류4충，순류4충，잡배류7충（필각、부남、필각병필진、밀정、새서、필남등），분류5충，포화방향경2충，불포화방향경2충，정류1충。유차가견，불동처리방식소득효모세포자용물을산을지췌취물공유21충성분，기여성분수진일보연구。
In order to analyze the differences of material composition in the cells yeasted by the adding different au-tolyzing solvent (NaCl and papain),the gas chromatography-mass spectrometry (GC-MS) technology were used to ana-lyze and examine the chemical composition of autolyzing solvent,and the structural identification was conducted by using computer retrieval techniques and the relative percentage of each component was calculated with chromatographic peak area normalization method.The results showed that 49 components were isolated from ethyl acetate extracts added NaCl and 28 components were identified,accounting to 92.14%of the total,in which there were three kinds of esters,five kinds of acids,four kinds of alcohol,six kinds miscellaneous rings (pyrrole,furan,pyrrole and pyrazine,pyrimidine,pyrazole, etc.),two kinds of phenols,three kinds of unsaturated aromatic hydrocarbons,five kinds of saturated aliphatic hydrocar-bons.The results showed that 49 components were isolated from ethyl acetate extracts added papaya protease and 27 components were identified, accounting to 91.006% of the total,of which there were two kinds of esters,four kinds of acids,four kinds of alcohols,seven kinds of heterocyclic (pyrrole,furan,pyrrole and pyrazine,pyrimidine,thiazole,pyran, etc.),five kinds of phenols,two kinds of saturated hydrocarbons,two kinds of the unsaturated aromatic hydrocarbon and one kind of nitriles.The results indicated that:the ethyl acetate extracts from the yeast cells through different treatment in-clude a total of 21 kinds of ingredients and the remaining ingredients need further study.