CAJ | 학술논문

目的研究铟暴露对外周血淋巴细胞线粒体ND1基因相对含量的影响.方法选取14例健康工人,抽取其静脉血,肝素钠抗凝,分离淋巴细胞并分成3管培养,其中2管分别加入终浓度为0.2和0.8 mmol/L氯化铟作为染毒组,另1管加入等体积的生理盐水作为对照组.培养72 h后,使用荧光定量PCR方法检测各组线粒体基因相对含量.结果 0.8 mmol/L氯化铟染毒组线粒体ND1基因相对含量明显高于对照组,差异有统计学意义(P＜0.05);0.8 mmol/L氯化铟染毒组线粒体ND1基因相对含量高于0.2 mmol/L氯化铟染毒组,差异有统计学意义(P＜0.05).结论高浓度的铟及其化合物暴露淋巴细胞的线粒体ND1基因相对含量升高,提示高浓度的铟及其化合物暴露可致DNA的氧化损伤增加.
목적 연구인폭로대외주혈림파세포선립체ND1기인상대함량적영향.방법 선취14례건강공인,추취기정맥혈,간소납항응,분리림파세포병분성3관배양,기중2관분별가입종농도위0.2화0.8 mmol/L록화인작위염독조,령1관가입등체적적생리염수작위대조조.배양72 h후,사용형광정량PCR방법검측각조선립체기인상대함량.결과 0.8 mmol/L록화인염독조선립체ND1기인상대함량명현고우대조조,차이유통계학의의(P＜0.05);0.8 mmol/L록화인염독조선립체ND1기인상대함량고우0.2 mmol/L록화인염독조,차이유통계학의의(P＜0.05).결론 고농도적인급기화합물폭로림파세포적선립체ND1기인상대함량승고,제시고농도적인급기화합물폭로가치DNA적양화손상증가.
Objective To study the effects of indium exposure on the relative content of mitochondrial ND1 gene in lymphocytes.Methods Venous blood was obtained from 14 healthy workers and anticoagulated with heparin.Blood lymphocytes were separated and divided into three tube cultures.For two tubes in the exposed group,indium chloride was added to final concentrations of 0.2 mmol/L and 0.8 mmol/L,respectively.For one tube in the control group,an equal volume of normal saline solution was added.After incubation for 72 h,the relative content of mitochondrial gene in each group was determined using quantitative real-time PCR.Results Lymphocytes exposed to 0.8 mmol/L indium chloride had a significantly higher relative content of mitochondrial gene than those exposed to 0.2 mmol/L indium chloride and those in the control group (P＜0.05,P＜0.05).Conclusion Lymphocytes exposed to a high concentration of indium and its compounds have an elevated relative content of mitochondrial ND1 gene,indicating increased oxidative DNA damage induced by exposure to a high concentration of indium and its compounds.