CAJ | 학술논문

目的：观察石见穿总甾醇对人胃癌细胞SGC－7901生长的影响，并探讨其可能的作用机制。方法用MTT法观察石见穿总甾醇对SGC－7901细胞增殖的影响，用荧光酶标仪检测对线粒体膜电位的影响，用流式细胞术检测细胞周期的影响。结果石见穿总甾醇能显著抑制SGC－7901细胞的增殖，具有剂量依赖性和时间依赖性，对SGC－7901细胞的线粒体膜电位没有显著影响，流式细胞术结果表明石见穿总甾醇处理后SGC－7901细胞以坏死为主。结论石见穿总甾醇对人胃癌细胞SGC－7901的增殖具有显著抑制作用，其作用机制可能是诱导细胞坏死有关。
목적：관찰석견천총치순대인위암세포SGC－7901생장적영향，병탐토기가능적작용궤제。방법용MTT법관찰석견천총치순대SGC－7901세포증식적영향，용형광매표의검측대선립체막전위적영향，용류식세포술검측세포주기적영향。결과석견천총치순능현저억제SGC－7901세포적증식，구유제량의뢰성화시간의뢰성，대SGC－7901세포적선립체막전위몰유현저영향，류식세포술결과표명석견천총치순처리후SGC－7901세포이배사위주。결론석견천총치순대인위암세포SGC－7901적증식구유현저억제작용，기작용궤제가능시유도세포배사유관。
s:Objective To observe the effect total sterol of Salvia Chinensis Benth.on growth of human gastric carcinoma line SGC-7901, and explore its possible mechanism.Methods To observe the effect of total sterol of Salvia Chinensis Benth.on proliferation of SGC-7901 cells by MTT method, and detect the mitochondrial membrane potential and cell cycle.Results The total sterol can inhibit the pro-liferation of SGC-7901 cells significantly in a dose and time dependent manner, and it has no notable effect on the mitochondrial membrane potential of SGC-7901 cells, and the results of flow cytometry showed the total sterol induce the SGC-7901 cells to necrosis.Conclusions The total sterol inhibit human gastric cancer cells SGC-7901 proliferation by induction of cell necrosis.