CAJ | 학술논문

目的：探讨Twist及YB-1基因在子宫颈癌组织中表达的临床病理意义及其与上皮间质转化标志物上皮细胞钙黏蛋白（ E-cadherin ）表达的相关性。方法选取2008年1月至2013年12月202例子宫颈组织标本，包括正常子宫颈组织50例、子宫颈上皮内瘤变（CIN）100例及宫颈鳞状细胞癌（SCC）52例，采用MaxVision法检测Twist、YB-1蛋白和E-cadherin，并对各组病例的表达差异进行卡方检验、相关性分析及回归模型分析。结果 Twist蛋白胞核高表达、YB-1蛋白胞质高表达与子宫颈上皮恶性转化、子宫颈癌的组织级别进展及转移能力提高相关。 Twist、YB-1蛋白高表达与E-cadherin低表达相关。回归分析提示Twist 蛋白的胞核表达是子宫颈癌进展的独立预测因子。结论 Twist和YB-1基因在子宫颈癌中过表达与CIN癌变、子宫颈癌侵袭及转移明显相关，其机制可能是通过（YB-1）-Twist-（E-cadherin）调节关系轴来实现上皮间质转化。 Twist和YB-1蛋白过表达可以预测子宫颈癌的恶性转化和转移。
목적：탐토Twist급YB-1기인재자궁경암조직중표체적림상병리의의급기여상피간질전화표지물상피세포개점단백（ E-cadherin ）표체적상관성。방법선취2008년1월지2013년12월202례자궁경조직표본，포괄정상자궁경조직50례、자궁경상피내류변（CIN）100례급궁경린상세포암（SCC）52례，채용MaxVision법검측Twist、YB-1단백화E-cadherin，병대각조병례적표체차이진행잡방검험、상관성분석급회귀모형분석。결과 Twist단백포핵고표체、YB-1단백포질고표체여자궁경상피악성전화、자궁경암적조직급별진전급전이능력제고상관。 Twist、YB-1단백고표체여E-cadherin저표체상관。회귀분석제시Twist 단백적포핵표체시자궁경암진전적독립예측인자。결론 Twist화YB-1기인재자궁경암중과표체여CIN암변、자궁경암침습급전이명현상관，기궤제가능시통과（YB-1）-Twist-（E-cadherin）조절관계축래실현상피간질전화。 Twist화YB-1단백과표체가이예측자궁경암적악성전화화전이。
Objective To investigate the clinicopathological significance of Twist and YB-1 up-regulation in cervical cancer , and to correlate the expression of the two genes with E-cadherin , a marker of epithelial-mesenchymal transition ( EMT).Methods A total of 202 tissue samples were collected during January 2008 to December 2013, including 50 cases of normal cervical tissues , 100 cases of cervical intraepithelial neoplasia ( CIN ) and 52 cases of squamous cell carcinoma ( SCC ).Twist, YB-1 and E-cadherin expression was investigated by MaxVision .Results Increased expression levels of Twist and YB-1 were found and correlated with the malignant transformation of cervical epithelium , histological progression and metastasis of cervical cancer.In addition, Twist and YB-1 overexpression was also associated with aberrant expression of E-cadherin.Regression analysis revealed that Twist expression was an independent factor for the histological progression of cervical cancer.Conclusions It is suggested that Twist and YB-1 overexpression is significantly linked to cervical cancer tumorigenesis and progression , likely related to EMT through ( YB-1 )-Twist-( E-cadherin ) pathway.Twist and YB-1 may be markers for determining the metastatic potential of cervical cancer.