CAJ | 학술논문

对从天然亚麻籽中提取的粗环肽的组分和氧化还原性质进行探讨。采用HPLC测定亚麻籽粗环肽中环肽C( CLC)、环肽E( CLE)、环肽D( CLD)、环肽A( CLA)的含量,并通过还原的方法减少粗环肽中的环肽种类,以制备环肽单体。结果表明,环肽样品峰分离度良好,线性范围10~150μg/mL(环肽A的为10~100μg/mL),相关系数0．9990~0．9997。所测4种环肽的含量分别为CLC 8．10%,CLE 7．66%,CLD 0．58%,CLA 35．18%。粗环肽经过氧化或还原反应之后,其种类均发生改变。经HPLC定性分析,氧化后剩余6种环肽,还原后的环肽仅剩下4种,通过与已知峰对比,得知4种环肽分别为环肽B、环肽L、环肽A和环肽M,在C18分析柱上分离良好,以此为理论依据,采用Microsorb 100-5 C18制备柱(240 mm ×21．4 mm),柱温30℃,流速5 mL/min,70%乙腈溶液等度洗脱,在50 min内可有效分离制备环肽单体。
대종천연아마자중제취적조배태적조분화양화환원성질진행탐토。채용HPLC측정아마자조배태중배태C( CLC)、배태E( CLE)、배태D( CLD)、배태A( CLA)적함량,병통과환원적방법감소조배태중적배태충류,이제비배태단체。결과표명,배태양품봉분리도량호,선성범위10~150μg/mL(배태A적위10~100μg/mL),상관계수0．9990~0．9997。소측4충배태적함량분별위CLC 8．10%,CLE 7．66%,CLD 0．58%,CLA 35．18%。조배태경과양화혹환원반응지후,기충류균발생개변。경HPLC정성분석,양화후잉여6충배태,환원후적배태부잉하4충,통과여이지봉대비,득지4충배태분별위배태B、배태L、배태A화배태M,재C18분석주상분리량호,이차위이론의거,채용Microsorb 100-5 C18제비주(240 mm ×21．4 mm),주온30℃,류속5 mL/min,70%을정용액등도세탈,재50 min내가유효분리제비배태단체。
The composition and oxidation and reduction properties of crude cyclolinopeptides extracted from natural flaxseed were studied. HPLC was employed to detect the contents of cyclolinopeptide C (CLC), cyclolinopeptide E(CLE), cyclolinopeptide D(CLD) and cyclolinopeptide A(CLA) in crude flaxseed cyclolinopeptides. Reduction was used to aid the separation of monomer as it could reduce the types of cyclolinopeptide in the crude cyclolinopeptides effectively. The results indicated that the cyclolin-opeptides were separated well, and the linear range and correlation coefficient were 10 -150 μg/mL (10-100 μg/mL for CLA) and 0. 999 0-0. 999 7, respectively. The contents of CLC, CLE, CLD and CLA in the crude cyclolinopeptides were 8. 10%, 7. 66%, 0. 58% and 35. 18% respectively. The cyclolinopeptide type in crude cyclolinopeptides could be transformed by oxidation or reduction re-action. According to the results of qualitative HPLC analysis, 6 cyclolinopeptides were left after oxidation, while only 4 cyclolinopeptides were left <br> after reduction and were respectively determined as CLB, CLL, CLA and CLM by comparing with the standard cyclolinopeptides. Besides, they were separated well by C18 column, based on this, singular cy-clolinopeptide was separated effectively in 50 min by preparative HPLC using Microsorb 100-5 C18 col-umn (240 mm × 21. 4 mm) under the conditions of column temperature 30℃, flow rate 5 mL/min and an isocratic elution of 70% acetonitrile solution.