CAJ | 학술논문

目的：研究低氧条件下胃癌细胞HIF1α及CD44的表达变化，并观察下调HIF1α表达对CD44表达的影响。方法以人胃癌细胞系SGC7901细胞为研究对象，分别于正常氧压及含1％ O2条件下传代培养1周。随后加入20 nm／L雷帕霉素培养72 h，CCK8法检测细胞活力，Transwell试验检测细胞侵袭能力，实时定量PCR及western blot检测CD44及HIF1α的mRNA及蛋白表达。结果与正常组对比，低氧条件下细胞增殖活性明显升高（P＜0．05），侵袭活性明显增强（P＜0．05），HIF1α及CD44基因的mRNA及蛋白表达水平明显升高（P＜0．01）。雷帕霉素处理后，不论在常氧及低氧条件下细胞活力均较对照组弱（P＜0．05），细胞侵袭活性明显下降（P＜0．05）。细胞HIF1α和CD44的mRNA及蛋白表达水平均明显降低（P＜0．01）。结论低氧条件下胃癌细胞SGC7901增殖及侵袭能力明显增强，下调细胞的HIF1α表达后，显著降低CD44的表达。由此可推测，低氧可能通过HIF1α调节CD44的表达，调控胃癌细胞增殖及侵袭潜能。
목적：연구저양조건하위암세포HIF1α급CD44적표체변화，병관찰하조HIF1α표체대CD44표체적영향。방법이인위암세포계SGC7901세포위연구대상，분별우정상양압급함1％ O2조건하전대배양1주。수후가입20 nm／L뢰파매소배양72 h，CCK8법검측세포활력，Transwell시험검측세포침습능력，실시정량PCR급western blot검측CD44급HIF1α적mRNA급단백표체。결과여정상조대비，저양조건하세포증식활성명현승고（P＜0．05），침습활성명현증강（P＜0．05），HIF1α급CD44기인적mRNA급단백표체수평명현승고（P＜0．01）。뢰파매소처리후，불론재상양급저양조건하세포활력균교대조조약（P＜0．05），세포침습활성명현하강（P＜0．05）。세포HIF1α화CD44적mRNA급단백표체수평균명현강저（P＜0．01）。결론저양조건하위암세포SGC7901증식급침습능력명현증강，하조세포적HIF1α표체후，현저강저CD44적표체。유차가추측，저양가능통과HIF1α조절CD44적표체，조공위암세포증식급침습잠능。
Objective To observe the change of expression of HIF1αand CD44 in human gastric cell line SGC7901 in hypoxia,and the effect of down-regulating HIF1αexpression on CD44 expression.Methods SGC7901 cells were cultured in 1%O2 condition for 1 week,and then treated with 20 nm/L rapamycin for 72 h.Cell viability was measured by CCK8 assay.Cell inva-sion was detected by Transwell invasion assay.Protein and mRNA expression of HIF1αand CD44 was detected by Western blot-ting and real-time RT-PCR.Results Compared with the control group,cell viability and invasion obviously increased in hypoxia (P<0.05),but decreased after treated with rapamycin (P<0.05).Protein and mRNA expression of HIF1αand CD44 obviously decreased in hypoxia (P<0.05),and inhibited by rapamycin (P<0.05).Conclusion In hypoxia,the proliferation and inva-sion of SGC7901 increased,and down-regulating the expression of HIF1αcan significantly inhibit CD44 expression.Therefore,it is possible that hypoxia can regulate the CD44 expression of gastric cancer cell via HIF1α,and regulate cell proliferation and inva-sion.