CAJ | 학술논문

目的：探讨PPARγ配体在肿瘤放射治疗中对肿瘤细胞的作用。方法采用RT-PCR方法检测CT-26细胞中是否有PPARγ表达。采用MTT方法检测PPARγ配体（吡格列酮）对CT-26细胞生长的影响。采用Western blot方法观察10 Gy X射线对CT-26细胞相关因子COX-2，PAI-1表达的影响，分析PPARγ配体（吡格列酮）对放射后CT-26细胞中COX-2、PAI-1表达变化的影响。结果 PCR结果显示CT-26细胞中有PPARγ表达。 MTT结果显示：吡格列酮可以降低CT-26细胞的存活率，10 Gy照射＋吡格列酮处理48 h时CT-26细胞存活率降低更为明显。10 Gy X射线照射CT-26细胞后Western blot方法检测结果显示：照射后COX-2表达水平升高，照射后24 h COX-2表达水平最高，吡格列酮对照射诱导的COX-2水平升高无明显影响。10 Gy X射线照射CT-26后Western blot方法检测结果显示：照射后24 hPAI-1表达水平明显增高，吡格列酮对照射诱导的PAI-1表达水平升高起降低作用。结论吡格列酮等噻唑烷二酮类药，作用于小鼠结肠癌细胞，在肿瘤放疗过程中可能发挥潜在的协同作用。
목적：탐토PPARγ배체재종류방사치료중대종류세포적작용。방법채용RT-PCR방법검측CT-26세포중시부유PPARγ표체。채용MTT방법검측PPARγ배체（필격렬동）대CT-26세포생장적영향。채용Western blot방법관찰10 Gy X사선대CT-26세포상관인자COX-2，PAI-1표체적영향，분석PPARγ배체（필격렬동）대방사후CT-26세포중COX-2、PAI-1표체변화적영향。결과 PCR결과현시CT-26세포중유PPARγ표체。 MTT결과현시：필격렬동가이강저CT-26세포적존활솔，10 Gy조사＋필격렬동처리48 h시CT-26세포존활솔강저경위명현。10 Gy X사선조사CT-26세포후Western blot방법검측결과현시：조사후COX-2표체수평승고，조사후24 h COX-2표체수평최고，필격렬동대조사유도적COX-2수평승고무명현영향。10 Gy X사선조사CT-26후Western blot방법검측결과현시：조사후24 hPAI-1표체수평명현증고，필격렬동대조사유도적PAI-1표체수평승고기강저작용。결론필격렬동등새서완이동류약，작용우소서결장암세포，재종류방료과정중가능발휘잠재적협동작용。
Objective To observe the effect of PPARγligand ( Pioglitazone) radiotherapy on mouse colonal cancer cells (CT-26).Methods After CT-26 cell was radiated by 10 Gy X-ray,RT-PCR was used to detect PPARγgene expression.MTT assay was used to assay the growth of CT-26 cell after the treatment by PPARγligand ( Pioglitazone) ,irradiation and the 2 com-bined.Western blot methods were used to measure the expression of COX-2,PAI-1 after CT-26 cell was irradiated by 10 Gy X-ray and treated by pioglitazone in different time.Results ①RT-PCR results indicated that PPARγmRNA was expressed in mouse colon cancer cell line.②MTT results showed that pioglitazone inhibits CT-26 growth,the influence was obvious after treated by pi-oglitazone +10 Gy X-ray radiation.③Western blot results indicated that expression of COX-2 and PAI-1 protein in mouse colon cancer cell line increased after irradiated by 10 Gy X-ray.Pioglitazone had no influence on the level of radiation-induce COX-2 overexpression,but decreased the level of radiation-induced PAI-1 overexpression after CT-26 cell was treated by 10 Gy X-ray ir-radiation for 24 h.After CT-26 cell was irradiated by X-ray,the expression of PAI-1,COX-2 increased.PPARγligand ( pioglata-zone) decreased the expression of radiation-induced PAI-1 but had no influence in the level of radiation-induce COX-2.Conclu-sion Thiazolidinedione,such as pioglatazone,plays potential benefit role to cancer patients during radiation.