医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2015年
24期
326-327
,共2页
复方芩连肠溶片%高效液相色谱法%盐酸小檗碱
複方芩連腸溶片%高效液相色譜法%鹽痠小檗堿
복방금련장용편%고효액상색보법%염산소벽감
fufang qinlian changrong tablets%HPLC%berberine hydrochloride
目的：建立以高效液相色谱法测定复方芩连肠溶片中盐酸小檗碱含量的方法。方法：以Waters XterraRP18为色谱柱，流动相为乙腈-水＝55：45，检测波长为265nm，流速为1ml·min-1，柱温为25℃，进样量是10μl。结果：盐酸小檗碱在3.071μg～24.568μg范围内进样量与峰面积呈良好的线性关系，平均回收率为100.2％，RSD=0.9％。结论：本方法简便、准确、专属性强，可用于该制剂的含量测定。
目的：建立以高效液相色譜法測定複方芩連腸溶片中鹽痠小檗堿含量的方法。方法：以Waters XterraRP18為色譜柱，流動相為乙腈-水＝55：45，檢測波長為265nm，流速為1ml·min-1，柱溫為25℃，進樣量是10μl。結果：鹽痠小檗堿在3.071μg～24.568μg範圍內進樣量與峰麵積呈良好的線性關繫，平均迴收率為100.2％，RSD=0.9％。結論：本方法簡便、準確、專屬性彊，可用于該製劑的含量測定。
목적：건립이고효액상색보법측정복방금련장용편중염산소벽감함량적방법。방법：이Waters XterraRP18위색보주，류동상위을정-수＝55：45，검측파장위265nm，류속위1ml·min-1，주온위25℃，진양량시10μl。결과：염산소벽감재3.071μg～24.568μg범위내진양량여봉면적정량호적선성관계，평균회수솔위100.2％，RSD=0.9％。결론：본방법간편、준학、전속성강，가용우해제제적함량측정。
Objective: To establish a method for the determination of berberine hydrochloride in fufang qinlian changrong tablets. Methods: HPLC was performed on a C18 column using acetonitrile-water (55:45) as the mobile phase at a flow rate of 1ml·min-1. The detection wavelength was at 265nm. The column temperature was 25℃ and the sample size w as 10μl.Results: The linear range of berberine hydrochloride was 3.071μg~24.568μg (r= 0.9999), the average recovery was 100.2%(RSD=0.9%).Conclusions: The method is simple, accurate, special and can be used for the quality control of fufang qinlian changrong tablets.
