CAJ | 학술논문

通过双环氧丁烷(diepoxybutane)诱变籼稻品种 IR64获得一个稳定遗传的红褐色斑点叶突变体 spl21(spot-ted-leaf 21)。大田条件下，突变体播种后约2周叶片上开始出现红褐色斑点，随后部分斑点融合，从叶尖开始发黄枯萎，并沿叶片两侧边缘向下扩散，严重时叶片大部分或整体枯死。突变体spl21与野生型IR64相比，其株高、穗长、有效穗数、实粒数、结实率和千粒重等农艺性状均显著降低。组织化学分析表明，叶片斑点处及周围有H2O2沉积。突变还导致叶绿素a、叶绿素b和类胡萝卜素含量极显著降低，叶片光合能力明显下降；此外，突变体中CAT、SOD、APX和可溶性蛋白含量均极显著降低， POD活性则极显著升高。遗传分析表明，突变体表型受1对隐性核基因控制。通过图位克隆法最终将该基因定位于第12染色体长臂下端介于InDel-8和RM28746之间约87 kb的区段内，暂名spl21(t)，本研究为该基因的克隆与功能研究奠定了基础。
통과쌍배양정완(diepoxybutane)유변선도품충 IR64획득일개은정유전적홍갈색반점협돌변체 spl21(spot-ted-leaf 21)。대전조건하，돌변체파충후약2주협편상개시출현홍갈색반점，수후부분반점융합，종협첨개시발황고위，병연협편량측변연향하확산，엄중시협편대부분혹정체고사。돌변체spl21여야생형IR64상비，기주고、수장、유효수수、실립수、결실솔화천립중등농예성상균현저강저。조직화학분석표명，협편반점처급주위유H2O2침적。돌변환도치협록소a、협록소b화류호라복소함량겁현저강저，협편광합능력명현하강；차외，돌변체중CAT、SOD、APX화가용성단백함량균겁현저강저， POD활성칙겁현저승고。유전분석표명，돌변체표형수1대은성핵기인공제。통과도위극륭법최종장해기인정위우제12염색체장비하단개우InDel-8화RM28746지간약87 kb적구단내，잠명spl21(t)，본연구위해기인적극륭여공능연구전정료기출。
Therice spotted-leaf 21mutant (spl21) was isolated from a diepoxybutane-induced IR64 mutant bank. Under field conditions, the red-brown spots appeared on the leaves of mutant seedlings in two weeks after sowing. Subsequently, a portion of spots merged and the leaf tips became yellowish, wilted and spread downwards along both edges of the leaf blade leading to the death of the whole leaf blade when the symptom was severe. Accumulation of H2O2 was detected in and around the spots. Major agronomic traits including plant height, length of panicle, number of panicles, number of filled grains, seed setting-rate, and 1000-grain weight were markedly affected in the mutant. The contents of chlorophylla,b, carotenoid and photosynthetic parame-ters were significantly reduced in the mutant as compared with the wild type. Furthermore, the activities of CAT, SOD, APX, and soluble protein contents were significantly lower than those of the wild type while the activity of POD was apparently higher than that of the wild type. The mutant trait was controlled by a single recessive nuclear gene, tentatively termedspl21(t), located on the long arm of chromosome 12. The population and data achieved in the present study would facilitate the isolation and functional analysis ofspl21(t).