湖北林业科技
湖北林業科技
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HUBEI FORESTRY SCIENCE AND TECHNOLOGY
2015年
4期
21-23
,共3页
蔡三山%陈京元%胡承辉%阳金华%傅云%刘刚
蔡三山%陳京元%鬍承輝%暘金華%傅雲%劉剛
채삼산%진경원%호승휘%양금화%부운%류강
PGPR%湿地松%火炬松%叶绿素含量
PGPR%濕地鬆%火炬鬆%葉綠素含量
PGPR%습지송%화거송%협록소함량
PGPR%Pinus elliottii%Pinus taeda%Chlorophyll content
将荧光假单胞菌 Q2-87、1M1-96、Pf-5和洋葱伯克霍尔德氏菌 C23等4个 PGPR 菌株分别于2014年4月和5月接种于火炬松和湿地松苗根部;并于当年8月份利用手持式叶绿素测定仪测定两种松苗针叶的 SPAD 值。结果如下:①接种菌株 Q2-87和1M1-96的火炬松苗针叶 SPAD 值显著高于清水对照;接种菌株 Pf-5与接种菌株 Q2-87和1M1-96的火炬松苗针叶 SPAD 值无显著性差异;②接种菌株 Q2-87和1M1-96的湿地松苗针叶 SPAD 值显著高于清水对照。根据本文的实验结果和参阅文献,推断菌株 Q2-87、1M1-96和 Pf-5的代谢产物2,4-DAPG 诱导火炬松和湿地松苗产生了系统诱导抗病性。
將熒光假單胞菌 Q2-87、1M1-96、Pf-5和洋蔥伯剋霍爾德氏菌 C23等4箇 PGPR 菌株分彆于2014年4月和5月接種于火炬鬆和濕地鬆苗根部;併于噹年8月份利用手持式葉綠素測定儀測定兩種鬆苗針葉的 SPAD 值。結果如下:①接種菌株 Q2-87和1M1-96的火炬鬆苗針葉 SPAD 值顯著高于清水對照;接種菌株 Pf-5與接種菌株 Q2-87和1M1-96的火炬鬆苗針葉 SPAD 值無顯著性差異;②接種菌株 Q2-87和1M1-96的濕地鬆苗針葉 SPAD 值顯著高于清水對照。根據本文的實驗結果和參閱文獻,推斷菌株 Q2-87、1M1-96和 Pf-5的代謝產物2,4-DAPG 誘導火炬鬆和濕地鬆苗產生瞭繫統誘導抗病性。
장형광가단포균 Q2-87、1M1-96、Pf-5화양총백극곽이덕씨균 C23등4개 PGPR 균주분별우2014년4월화5월접충우화거송화습지송묘근부;병우당년8월빈이용수지식협록소측정의측정량충송묘침협적 SPAD 치。결과여하:①접충균주 Q2-87화1M1-96적화거송묘침협 SPAD 치현저고우청수대조;접충균주 Pf-5여접충균주 Q2-87화1M1-96적화거송묘침협 SPAD 치무현저성차이;②접충균주 Q2-87화1M1-96적습지송묘침협 SPAD 치현저고우청수대조。근거본문적실험결과화삼열문헌,추단균주 Q2-87、1M1-96화 Pf-5적대사산물2,4-DAPG 유도화거송화습지송묘산생료계통유도항병성。
Four PGPR strains including Pseudomonas fluorescens Q2-87,1M1-96,Pf-5 and Burkholderia cepacia C23, were inoculated on Pinus taeda and P .elliottii in April and May,2014,respectively;The SPAD values of pine seedlings were measured using SPAD 502 PLUS in August,2014.The results were as follows:①The SPAD values of the needle leaves of P .taeda seedling inoculated with Q2-87 and 1M1-96 were significantly higher than that of P .taeda seedling in-oculated with water;The SPAD Values of P .taeda seedlings inoculated with Pf-5 had no significant difference with that of P .taeda seedlings inoculated with Q2-87 and 1M1-96;②The SPAD values of the needle leaves of P .elliottii seedling inoculated with Q2-87 and 1M1-96 were significantly higher than that of P .elliottii seedling inoculated with water.Ac-cording to the experimental results and the references,we estimate that ISR was induced by 2,4-DAPG in the two kinds of pine inoculated with Q2-87,1M1-96 and Pf-5.