CAJ | 학술논문

大豆胞囊线虫(soybean cyst nematode, SCN)严重危害世界大豆生产,Rhg4(resistance toHeterodera glycines 4)是控制大豆SCN抗性的2个主效位点之一。本研究针对Rhg4(GmSHMT)上的2个单核苷酸多态性(single nucleotide polymorphisms, SNP)位点开发了快速、经济、简便易行的CAPS (Rhg4-389)和dCAPS标记(Rhg4-1165),并用开发的2个标记鉴定了以大豆胞囊线虫应用核心种质为主的193份代表性抗感种质。结果表明, Rhg4-389和Rhg4-1165位点间存在显著连锁不平衡(P=0.0001, r2=0.87),可形成4种单倍型。Rhg4-389-G/Rhg4-1165-T和Rhg4-389-C/Rhg4-1165-A为优势单倍型,稀有单倍型Rhg4-389-G/Rhg4-1165-A和Rhg4-389-C/Rhg4-1165-T是在中国抗源中新发现的单倍型。结合193份种质对SCN 3号小种抗性鉴定分析发现, Rhg4-389-G和Rhg4-1165-T主要存在于抗病种质,它们形成的单倍型对抗病种质鉴定效率可达94.1%。本研究开发了可用于辅助大豆 SCN 抗性鉴定且方便育种家利用的CAPS/dCAPS标记,且用其摸清了应用核心种质等重要抗源在Rhg4位点的“本底”,为育种家有效利用这些优异抗源提供了重要信息。
대두포낭선충(soybean cyst nematode, SCN)엄중위해세계대두생산,Rhg4(resistance toHeterodera glycines 4)시공제대두SCN항성적2개주효위점지일。본연구침대Rhg4(GmSHMT)상적2개단핵감산다태성(single nucleotide polymorphisms, SNP)위점개발료쾌속、경제、간편역행적CAPS (Rhg4-389)화dCAPS표기(Rhg4-1165),병용개발적2개표기감정료이대두포낭선충응용핵심충질위주적193빈대표성항감충질。결과표명, Rhg4-389화Rhg4-1165위점간존재현저련쇄불평형(P=0.0001, r2=0.87),가형성4충단배형。Rhg4-389-G/Rhg4-1165-T화Rhg4-389-C/Rhg4-1165-A위우세단배형,희유단배형Rhg4-389-G/Rhg4-1165-A화Rhg4-389-C/Rhg4-1165-T시재중국항원중신발현적단배형。결합193빈충질대SCN 3호소충항성감정분석발현, Rhg4-389-G화Rhg4-1165-T주요존재우항병충질,타문형성적단배형대항병충질감정효솔가체94.1%。본연구개발료가용우보조대두 SCN 항성감정차방편육충가이용적CAPS/dCAPS표기,차용기모청료응용핵심충질등중요항원재Rhg4위점적“본저”,위육충가유효이용저사우이항원제공료중요신식。
Soybean cyst nematode (SCN,Heterodera glycinesIchinohe) is one of the most destructive diseases which largely suppressed soybean production worldwide.Rhg4 was one of two major resistant loci for SCN. In this study two nonsynonymous SNPs (Rhg4-389-G/C and Rhg4-1165-T/A) inGmSHMT were deduced to confer SCN resistance or susceptibility. One CAPS marker (Rhg4-389) and one dCAPS marker (Rhg4-1165) with the advantages of rapid, economic, simple and easy way to use were developed based on two nonsynonymous SNPs (Rhg4-389-G/C and Rhg4-1165-T/A), respectively. Then, a total of 193 soy-bean cultivars mainly from SCN-applied-core collection were genotyped by markers Rhg4-389 and Rhg4-1165. Linkage disequi-librium (LD) analysis indicated pairwise of Rhg4-389 and Rhg4-1165 was in significant LD (P-value = 0.0001) withr2 of 0.87. Among four haplotypes detected in this panel, Rhg4-389-G/Rhg4-1165-T and Rhg4-389-C/Rhg4-1165-A were predominant; while both Rhg4-389-G/Rhg4-1165-A and Rhg4-389-C/Rhg4-1165-T were rare, which only discovered in the resistant cultivars from China. Rhg4-389-G and Rhg4-1165-T alleles mainly occurred in resistant cultivars, and almost coincided with Rhg4-389-G/ Rhg4-1165-T haplotype. Of 101 resistant cultivars with Rhg4-389-G/Rhg4-1165-T haplotype, 94.1% (95) were resistant to SCN. In this study, we not only developed convenient CAPS/dCAPS markers for marker assisted selection (MAS) breeding, but also dissected the genetic architecture ofRhg4 locus among SCN-applied-core collection. Hopefully, this study will provide helpful information for improving the utilization efficiency of resistant resources in soybean breeding.