CAJ | 학술논문

采用0.05%,0.1%和0.2%3个浓度秋水仙素对根茎型清水紫花苜蓿种子进行4 h 处理,在幼苗3~4叶期进行干旱胁迫,通过测定胁迫0、3、6、9及12 d 复水后叶片相对含水量(RWC)、丙二醛(MDA)、游离脯氨酸(Pro)及叶绿素含量,研究不同浓度处理下清水紫花苜蓿对干旱胁迫的适应能力。结果表明：随着干旱胁迫时间的延长,清水紫花苜蓿叶片 RWC 及叶绿素含量呈现降低趋势,且均在0.2%处理下降低最小；而 MDA 及 Pro 含量呈不同程度增加,但 MDA 含量在0.1%处理下积累最少,而 Pro 含量在0.2%处理下积累最多。同时,对各处理植株的叶片 RWC、Pro 含量、MDA 含量及叶绿素含量4个生理生化指标进行综合分析,得出不同浓度秋水仙素处理的根茎型清水紫花苜蓿植株抗旱性强弱顺序为：0.1%>0.05%>0.2%>CK。
채용0.05%,0.1%화0.2%3개농도추수선소대근경형청수자화목숙충자진행4 h 처리,재유묘3~4협기진행간한협박,통과측정협박0、3、6、9급12 d 복수후협편상대함수량(RWC)、병이철(MDA)、유리포안산(Pro)급협록소함량,연구불동농도처리하청수자화목숙대간한협박적괄응능력。결과표명：수착간한협박시간적연장,청수자화목숙협편 RWC 급협록소함량정현강저추세,차균재0.2%처리하강저최소；이 MDA 급 Pro 함량정불동정도증가,단 MDA 함량재0.1%처리하적루최소,이 Pro 함량재0.2%처리하적루최다。동시,대각처리식주적협편 RWC、Pro 함량、MDA 함량급협록소함량4개생리생화지표진행종합분석,득출불동농도추수선소처리적근경형청수자화목숙식주항한성강약순서위：0.1%>0.05%>0.2%>CK。
This paper used four treatments of different concentrations of colchicine(0.05%、0.1%、0.2%)to treat seeds of Medicago sativa cv.Qingshui for 4 hours and conducted drought stress when the seedlings was in 3~4 leaf stage.The contents of RWC,MDA,PRO and Chlorophyll in leaves under drought stress were meas-ured on 0 d、3 d、6 d、9 d and rewater after 12 d.The results showed that the contents of RWC,and Chlorophyll of plant leaves were decreasing,and the lowest values were in the colchicine with 0.2%;MDA and Pro were in-creasing.However,MDA had a least accumulation under the treatment with 0.1%,and Pro had a most accumu-lation under the treatment with 0.2%.Meanwhile,based on the comphensive analyse of RWC,MDA,PRO and Chlorophyll,the rank of colchicine concentrations on the drought resistance of Qingshui alfalfa was:0.1% >0.05%>0.2%>CK.