CAJ | 학술논문

在室内条件下,用不同浓度的长枝木霉孢子悬浮液对白三叶种子进行处理,研究了其种子发芽率、根芽长及抗逆性酶活变化,以及长枝木霉对白三叶种子的促生作用及抗逆性的影响。结果表明,长枝木霉孢子悬浮液浓度在5.0×105个/mL~1.0×107个/mL 时能促进白三叶种子活性,在孢子浓度为1.0×106个/mL 时,发芽率为93%,与对照80%相比提高了16.25%；胚根长1.93 cm,与对照1.26 cm 相比增长了53.1%；胚芽长1.33 cm,与对照1.04 cm 相比增长了27.8%。抗逆性结果表明,长枝木霉孢子悬浮液能够提高白三叶种子抗病害及不良环境的能力,降低 MDA 含量,提高 PAL、POD 的活性。其中,孢子悬浮液浓度为5.0×105个/mL 时,PAL 活性最高,为2.157 U/(g·min)FW；孢子浓度为1.0×106个/mL 时 POD 的活性最高,为29.875 U/g FW；孢子悬浮液浓度5.0×105个/mL 时,MDA含量最低,为1.012μmol/g FW。
재실내조건하,용불동농도적장지목매포자현부액대백삼협충자진행처리,연구료기충자발아솔、근아장급항역성매활변화,이급장지목매대백삼협충자적촉생작용급항역성적영향。결과표명,장지목매포자현부액농도재5.0×105개/mL~1.0×107개/mL 시능촉진백삼협충자활성,재포자농도위1.0×106개/mL 시,발아솔위93%,여대조80%상비제고료16.25%；배근장1.93 cm,여대조1.26 cm 상비증장료53.1%；배아장1.33 cm,여대조1.04 cm 상비증장료27.8%。항역성결과표명,장지목매포자현부액능구제고백삼협충자항병해급불량배경적능력,강저 MDA 함량,제고 PAL、POD 적활성。기중,포자현부액농도위5.0×105개/mL 시,PAL 활성최고,위2.157 U/(g·min)FW；포자농도위1.0×106개/mL 시 POD 적활성최고,위29.875 U/g FW；포자현부액농도5.0×105개/mL 시,MDA함량최저,위1.012μmol/g FW。
The effect of Trichoderma longibrachiatum on seeds growth and resistance of clover was studied by measuring the germination rates,root length and resistance of clover treated with different spores concentra-tions of T.longibrachiatum.The results showed that the vigor of clover seeds was increased after treated with the concentration of 5.0 × 10 5 to 1.0 × 10 7 spores/mL of T .longibrachiatum.The germination rates,root length,shoot length were 93%,1.93 cm and 1.33 cm after treated with the concentration of 1.0 × 10 6 spores/mL,and which were increased by 1 6.25%,53.1% and 27.8% compared to control(80%,1.26 cm,1.04 cm),respectively.The spore suspension of T .longibrachiatum could enhance the ability of seeds resistance to plant disease and unfavorable environment.The activity of phenylalanine(PAL)and peroxidase(POD)were in-creased after treated with the spore suspension,but the activity of methane dicarboxylic aldehyde(MDA)was decreased.The maximum activity of PAL and POD were 2.157 U/g(FW)·min and 29.875 U/g(FW)while the spore suspension concentrations were 5.0×10 5 and 1.0×10 6 spores/mL,respectively.The minimum activity of MDA was 1.012μmol/g(FW)treated with 5.0×10 6 spores/mL.