北京生物医学工程
北京生物醫學工程
북경생물의학공정
BEIJING BIOMEDICAL ENGINEERING
2015年
4期
394-397,408
,共5页
原子荧光光谱%微波消解%透明质酸钠凝胶%砷%汞%含量
原子熒光光譜%微波消解%透明質痠鈉凝膠%砷%汞%含量
원자형광광보%미파소해%투명질산납응효%신%홍%함량
atomic fluorescence spectrometry%microwave digestion%sodium hyaluronate gel%arsenic%mercury%content
目的：建立利用原子荧光光谱法检测注射用透明质酸钠凝胶中砷和汞含量的方法。方法首先采用微波消解仪使样品完全消解，将样品消解液定容后，利用原子荧光光谱仪分析样品检验液中砷和汞的含量。通过标准曲线、检出限、精密度和加标回收率证明该方法的有效性。结果此方法中砷和汞的浓度分别在1~50 ng / mL 和0.1~10 ng / mL 范围内与荧光强度成良好的线性关系，相关系数分别是0.9999%和0.9997%。砷和汞的检出限分别是0.0105 ng / mL 和0.0032 ng / mL，精密度 RSD 均<5%，平均加标回收率分别是95.2%和100.2%。此面部注射用透明质酸钠凝胶样品中砷含量低于检出限（<0.49 ng / g），汞含量为7.22 ng / g。结论用于测定注射用透明质酸钠凝胶中砷和汞含量的微波消解-原子荧光光谱法具有良好的线性范围、高灵敏度和准确度、重复性好等优点。
目的：建立利用原子熒光光譜法檢測註射用透明質痠鈉凝膠中砷和汞含量的方法。方法首先採用微波消解儀使樣品完全消解，將樣品消解液定容後，利用原子熒光光譜儀分析樣品檢驗液中砷和汞的含量。通過標準麯線、檢齣限、精密度和加標迴收率證明該方法的有效性。結果此方法中砷和汞的濃度分彆在1~50 ng / mL 和0.1~10 ng / mL 範圍內與熒光彊度成良好的線性關繫，相關繫數分彆是0.9999%和0.9997%。砷和汞的檢齣限分彆是0.0105 ng / mL 和0.0032 ng / mL，精密度 RSD 均<5%，平均加標迴收率分彆是95.2%和100.2%。此麵部註射用透明質痠鈉凝膠樣品中砷含量低于檢齣限（<0.49 ng / g），汞含量為7.22 ng / g。結論用于測定註射用透明質痠鈉凝膠中砷和汞含量的微波消解-原子熒光光譜法具有良好的線性範圍、高靈敏度和準確度、重複性好等優點。
목적：건립이용원자형광광보법검측주사용투명질산납응효중신화홍함량적방법。방법수선채용미파소해의사양품완전소해，장양품소해액정용후，이용원자형광광보의분석양품검험액중신화홍적함량。통과표준곡선、검출한、정밀도화가표회수솔증명해방법적유효성。결과차방법중신화홍적농도분별재1~50 ng / mL 화0.1~10 ng / mL 범위내여형광강도성량호적선성관계，상관계수분별시0.9999%화0.9997%。신화홍적검출한분별시0.0105 ng / mL 화0.0032 ng / mL，정밀도 RSD 균<5%，평균가표회수솔분별시95.2%화100.2%。차면부주사용투명질산납응효양품중신함량저우검출한（<0.49 ng / g），홍함량위7.22 ng / g。결론용우측정주사용투명질산납응효중신화홍함량적미파소해-원자형광광보법구유량호적선성범위、고령민도화준학도、중복성호등우점。
Objective To establish one method of the determination of arsenic and mercury in injectable sodium hyaluronate gel by atomic fluorescence spectrometry. Methods The mercury and arsenic contents in samples were determined by atomic fluorescence spectrometry after microwave digestion. Results The concentrations of arsenic and mercury kept good and wide linear relationships with fluorescence intensity,and the coefficient correlations were 0. 9999% and 0. 9997%,respectively. The detection limits of arsenic and mercury in this method were 0. 0105 ng / mL and 0. 0032 ng / mL,respectively. Precisions both showed RSD <0. 5% . The average adding standard recovery rates of arsenic and mercury were 95. 2% and 100. 2%, respectively. The content of arsenic was below the detection limit( <0. 49 ng / g)while the content of mercury was 7. 22 ng / g. Conclusions This microwave digestion-atomic fluorescence spectrometry applied to determine the mercury and arsenic contents in injectable sodium hyaluronate gel had the advantages of high accuracy and precision,good linear relationship and repeatability.
